摘要
苯酚和喹啉单基质降解菌在实际应用中局限性较大,筛选共基质条件下具有生物降解活性的菌株更具有实际价值。从焦化厂废水处理系统的污泥中经过驯化、分离,筛选出一株能同时降解苯酚和喹啉的菌株KD1,根据形态特征、生理生化和16S rDNA序列等分析,鉴定其为粪产碱杆菌(Alcaligenes faecalis)。降解条件优化表明,菌株KD1有较好的酸碱度适用性,在接菌量为10%、初始pH值为7.0、温度35℃、振荡器转速150 r/min时,对苯酚和喹啉的降解效率最高;氮源能影响菌株KD1对苯酚的降解,而共基质下菌株KD1能协同降解苯酚和喹啉。菌株KD1能完全降解700 mg/L的苯酚和400 mg/L的喹啉,降解过程均呈零级反应特征,其在不同浓度的苯酚和喹啉中的生长符合Haldane底物抑制模型,抑制浓度分别为293 mg/L和229 mg/L。固定化研究表明,通过包埋和吸附制备的新型固定化载体能提高菌株KD1对苯酚和喹啉共基质的降解效率。
The strains that can only degrade phenol or quinoline are limited in practical application.It is more practical to select the tolerant strains which can degrade the compound pollutants.A phenol-quinoline degrading strain KD1 was isolated from activated sludge in a coke-plant wastewater treatment system.KD1 was identified as Alcaligenes faecalis on the basis of morphological characteristics,physiological and biochemical characteristics and the analysis of its 16 S rDNA sequence.The optimal degradation conditions showed that strain KD1 had good pH applicability,and the optimal conditions were as follow:inoculation volume 10%,rotation rate 150 r/min,culture temperature 30℃,initial pH value 7.0 respectively.Nitrogen source can affect the degradation of phenol by strain KD1,while strain KD1 can synergistically degrade phenol and quinoline under co-substrate.The degradation reaction of phenol and quinoline can be described with zero order kinetic equation within 700 mg/L phenol and 400 mg/L respectively.The growth kinetics of Strain KD1 could be described with Haldanes inhibition model,and the inhibition concentrations of it were 293 mg/L and 229 mg/L,respectively.The new immobilized carrier prepared by embedding and adsorption can improve the degradation efficiency of co-substrate pollutants.
作者
赵乾程
苏凯文
邱子梁
王庆宇
于彩虹
Zhao Qiancheng;Su Kaiwen;Qiu Ziliang;Wang Qingyu;Yu Caihong(School of Chemical and Environmental Engineering,China University of Mining and Technology-Beijing,Beijing 100083,China)
出处
《矿业科学学报》
CSCD
2022年第2期247-256,共10页
Journal of Mining Science and Technology
基金
国家自然科学基金(41977029)
中央高校基本科研业务费专项资金(2009QH11)。
关键词
苯酚
喹啉
共降解
降解特性
固定化
phenol
quinoline
co-degradation
degradation characteristics
immobilization
