摘要
目的探讨单味中药细辛对膝骨性关节炎(KOA)模型膝周不同组织炎症因子的影响。方法将36只健康雄性新西兰大白兔采用随机数字表分为6组,即:空白组(A)、模型组(B)、对照组(C)、低剂量细辛组(D)、中剂量细辛组(E)、高剂量细辛组(F),每组6只。除A组外,均采用本课题组改良方法复制KOA模型,即造模开始第1、4、7天向右后肢关节腔内注射木瓜蛋白酶水溶液。第8天开始,麻醉、绑定、固定造模肢足底,调节改良装置转速为120次/min,行程15cm,牵拉足部,使其造模肢被动屈伸,牵拉肌肉。每天1次,每次2 h,共7天。造模14天后,于第15天进行干预,D、E、F组分别灌服不同浓度的细辛水溶液(根据药典剂量换算低、中、高剂量分别为0.14、0.28、0.7 g/kg),1次/天,共5天。C组给予吲哚美辛片水溶液(根据药典换算单次剂量为0.03g/kg)灌胃,1次/天,共5天。A、B组给予生理盐水灌胃。观察各组兔行为学变化及膝关节肿胀程度,HE染色观察各组图不同组织的病理改变,检测各组关节腔积液、滑膜、软骨、肌肉的IL-1β、肿瘤坏死因子(TNF-α)含量及肝、肾功能。结果B组各组织中炎症因子IL-1β、TNF-α水平显著高于A组(P<0.05,P<0.01);与B组比较,D、E、F组滑膜、软骨、肌肉组织中IL-1β、TNF-α水平均显著降低(P<0.05,P<0.01)。结论细辛有着很强的抗炎作用,其抗炎作用在肌肉、软骨、滑膜中无明显差异。
Objective To observe anti-inflammatory effects of Asarum Sieboldii(AS)on different tissues around the knee of knee osteoarthritis(KOA)rabbits.Methods Totally 36 healthy male New Zealand white rabbits were randomly divided into six groups by random number table,i.e.,the blank group(A),the model group(B),the control group(C),the low dose AS Group(D),the middle dose AS Group(e),and the high dose AS Group(F),6 in each group.Except group A,KOA model was established and duplicated by modified methods of this project.The right hind limb joint was injected with papain solution on day 1,4,and 7.Starting from the 8th day,anesthesia,binding,and fixing the molded limb plantar,adjusting the modified device rotational speed to 120 times/min,stroke 15 cm,pulling the foot,making the molded limb passive flexion and extension,pulling the muscle,once a day for 2 h for a total of 7 days.After 14 days after modeling,intervention was carried out on the 15th day,and AS water solution at 0.14,0.28,0.7 g/kg was administered to those in Group D,E,F by gastrogavage,once a day for 5 successive days.Indomethacin Tablets water solution(0.03 g/kg)was administered to those in group C by gastrogavage.Normal saline was given to those in group A and B by gastrogave.The changes of behavior and swelling degree of knee joint in each group were observed.The pathological changes of different tissues in each group were observed by HE staining.The IL-1βand TNF-αcontents of inflammatory factors in joint cavity effusion,synovium,cartilage,muscle,liver and kidney functions were detected.Results The levels of IL-1βand TNF-αwere significantly higher in group B than in group A(P<0.05,P<0.01).Compared with group B,the levels of IL-1βand TNF-αin the synovium,cartilage,and muscle tissues significantly decreased in group D,E,F(P<0.05,P<0.01).Conclusion AS had strong anti-inflammatory effects,and its anti-inflammatory effects on muscle,cartilage,or synovium had no significant difference.
作者
钟欢
裴鑫
周予婧
常小荣
刘密
王小军
邵湘宁
谭晓文
ZHONG Huan;PEI Xin;ZHOU Yu-jing;CHANG Xiao-rong;LIUMi;WANG Xiao-jun;SHAO Xiang-ning;TAN Xiao-wen(College of Acu-moxibustion and Massage,Hunan University of Chinese Medicine,Changsha,410007;Department of Cardiology,Xiangxi Hospital of Chinese Medicine of Tujia and Miao Ethnic Group,Xiangxi Tujia and Miao Autonomous Prefecture Hunan,416000;College of Integrated Chinese and Western Medicine,Hunan University of Traditional Chinese Medicine,Changsha,410007;Second Hospital of Integrated Traditional Chinese and Western Medicine,Hunan University of Chinese Medicine,Hunan,410300)
出处
《中国中西医结合杂志》
CAS
CSCD
北大核心
2021年第12期1496-1501,共6页
Chinese Journal of Integrated Traditional and Western Medicine
基金
国家自然科学基金青年项目(No.81904097)
湖南省自然科学基金项目(No.2020JJ5430)
湖南省中医药科研计划(No.201963)
湖南省大学生创新创业训练计划项目(No.201707010506)。
关键词
细辛
抗炎作用
膝骨性关节炎
滑膜
肌肉
软骨
中药
Asarum Sieboldii
anti-inflammatory effect
knee osteoarthritis
synovium
muscle
cartilage
Chinese herbs
