摘要
分别从体液免疫、细胞免疫和单核-巨噬细胞活性测定研究了动物双歧杆菌(B0,商业菌)、乳双歧杆菌(BL-99)、副干酪乳酪杆菌(LPC0,商业菌)和副干酪乳酪杆菌(ET-22)4株益生菌对小鼠免疫功能的调节作用。体液免疫实验发现,4株益生菌均能显著影响小鼠体液免疫。在脾淋巴细胞转化和迟发型免疫反应实验中,B0组的OD数值显著高于对照组(p<0.01),B0,BL-99和LPC0,在迟发型免疫反应中,足趾肿胀度显著高于对照组(p<0.05),细胞免疫实验表明,B0、BL-99和LPC0均具有调节小鼠免疫作用。体液免疫实验表明,B0、BL-99、LPC0、ET-22在抗体生成细胞以及半数溶血值均有显著影响调节作用(p<0.05)。单核巨噬细胞功能实验表明,BL-99和LPC0能够显著调节碳廓清以及巨噬细胞吞噬荧光微球的能力。最后,BL-99、LPC0以及ET-22均对NK细胞活性有显著性影响(p<0.05)。结果表明,B0、BL-99、LPC0和ET-22均可以通过影响单核-巨噬细胞功能进行免疫调节,因实验方法参照《保健食品功能评价技术规范》,且试验结果符合评价程序中"具有增强免疫力"的评价标准,因此可判断,4株菌均具有增强免疫力的功能。
In this experiment, the modulatory effects of 2 bifidobacteria and 2 Lactobacillus paracasei(Lacticaseibacilli paracasei) strains, Bifidobacterium animalis(B0, commercial strain), Bifidobacterium lactis(BL-99, owned by Yili), Lactobacillus paracasei(Lacticaseibacilli paracasei)(LPC0, commercial strain) and Lacticaseibacilli paracasei(ET-22, owned by Yili) on immunity were investigated from humoral immunity, cellular immunity and monocyte-macrophage activity assays, respectively. Humoral immunity experiments revealed that all four strains significantly affected humoral immunity in mice. In the splenic lymphocyte transformation and delayed allergic reaction experiments, the OD values of B0 group were significantly higher than those of the control group(p<0.01), B0, BL-99 and LPC0, and the paw-swelling was significantly higher than that of the control group in the delayed allergic reaction(p<0.05). Cellular immunity experiments showed that B0, BL-99 and LPC0 all had a modulating effect on immunity in mice. Humoral immunity experiments showed that B0, BL-99, LPC0, and ET-22 had a significant effect on the regulation of antibody-producing cells as well as hemolytic values(p<0.05). Mononuclear macrophage function experiments showed that BL-99 and LPC0 significantly modulated carbon clearance rate as well as the ability of macrophages to phagocytose fluorescent microspheres. Finally, BL-99, LPC0, and ET-22 all had a significant effect on NK cell activity(p<0.05). These results showed that B0,BL-99, LPC0 and ET-22 could regulate immunity by influencing monocyte-macrophage function. Since the experimental was performed according to the evaluation procedure“Technical Specification for Functional Evaluation of Health Food”in China, and the test results met the evaluation criteria of "able to enhance immunity", all fours strains are potential probiotic ingredients for enhancing immunity.
作者
赵雯
刘伟贤
张海斌
马国文
尹小静
洪维鍊
ZHAO Wen;LIU Weixian;ZHANG Haibin;MA Guowen;YIN Xiaojing;HONG Weilian(Inner Mongolia Yili Industrial Group Co.Ltd.,Hohhot 010110,China)
出处
《中国乳品工业》
CAS
北大核心
2021年第11期13-18,共6页
China Dairy Industry
