摘要
为筛选出能在低温条件下高效降解纤维素的菌株,提高秸秆在低温条件下纤维素的降解速度,以新疆寒冷地区腐木为试验材料,对低温纤维素降解菌进行筛选,在4℃条件下筛选得到4株可在低温下生长且具有纤维素降解作用的真菌,通过形态学和分子生物学的方法对低温菌进行鉴定,分别为产黄青霉(Penicillium chrysogenum)、桔绿木霉(Trichoderma citrinoviride)2株、脉纹孢菌(Neurospora sitophila);耐冷试验表明,筛选获得的菌株都为耐冷菌。通过对4株低温菌产酶特性进行研究,结果表明,菌株产纤维素酶的最佳培养时间为9 d,培养基最适初始pH值为7,最佳温度为25℃,最佳接种量为5%。秸秆降解试验表明,筛选获得的4株真菌对秸秆具有降解能力,对玉米秸秆降解效果最好,酵解率都在40%以上。
In order to screen out the strains which can degrade cellulose efficiently at low temperature and improve the degradation rate of cellulose under low temperature,in this study,rotten wood in cold area of Xinjiang was used as experimental material,and the low-temperature cellulose degrading bacteria were screened.Four strains of fungi which could grow at low temperature and degrade cellulose were screened at 4℃.Morphological and molecular biological methods were used for identification of low temperature strains.They were Penicillium chrysogenum,two Trichoderma citrinoviride strains and Neurospora sitophila.The cold tolerance test showed that all the strains were cold resistant.Studies on the characteristics of enzyme production of 4 low-temperature bacteria showed that the optimal culture time for cellulase production was 9 d,the optimal initial pH of the culture medium was 7,the optimal temperature was 25℃,and the optimal inoculation amount was 5%.The straw degradation test showed that these four strains of fungi had ability to degrade the straw,and had the best degradation effect on the corn straw with the fermentation rate above 40%.
作者
冯欣欣
李凤兰
徐永清
李磊
贺付蒙
冯艳忠
袁强
刘娣
FENG Xinxin;LI Fenglan;XU Yongqing;LI Lei;HE Fumeng;FENG Yanzhong;YUAN Qiang;LIU Di(School of Life Sciences, Northeast Agricultural University, Harbin 150030, China;Heilongjiang Academy of Agricultural Sciences, Harbin 150030, China)
出处
《浙江农业学报》
CSCD
北大核心
2021年第8期1468-1476,共9页
Acta Agriculturae Zhejiangensis
基金
国家自然基金青年科学基金(31201470)
国家科技部科技基础性工作专项(2014FY111000)
哈尔滨市应用科技研究与开发项目(2015RAQXJ021)。
关键词
纤维素
降解菌
低温
菌株鉴定
秸秆降解
cellulose
degrading bacteria
low temperature
strain identification
straw degradation
