摘要
从已经腐败的笋子烧牛肉中用稀释涂布平板法和划线分离法分离纯化出纯种菌株,根据生理生化试验结果,查阅《伯杰细菌鉴定手册》与《常见细菌系统鉴定手册》对菌株进行初步鉴定,再经16S rRNA的鉴定,将其鉴定到种。结果表明,笋子烧牛肉中的优势腐败菌为金黄色葡萄球菌、苏云金芽孢杆菌、暹罗芽孢杆菌和枯草芽孢杆菌。
The pure strains were isolated and purified from the rotten bamboo shoots and beef by dilution coating plate method and line separation method.According to the results of physiological and biochemical experiments,and the Berger's Bacterial Identification Manual and Manual for Systematic Identification of Common Bacteria,the strain was preliminarily identified.And they were further identified by comparing their 16S rRNA and constructing phylogenetic tree.The results showed that the dominant spoilage bacteria were Staphylococcus aureus,Bacillus thuringiensis,Bacillus siamensis and Bacillus subtilis.
作者
甯雨荞
田靓
袁先铃
刘永明
NING Yuqiao;TIAN Liang;YUAN Xianling;LIU Yongming(School of Biological Engineering,Sichuan University of Science and Engineering,Zigong,Sichuan 643000,China)
出处
《农产品加工》
2021年第8期51-55,共5页
Farm Products Processing
基金
四川理工学院大学生创新创业训练计划项目(201810622076)
四川理工学院人才引进项目(2017RC75)。
