摘要
为揭示不同肥料配施尿素对茶园土壤氮转化酶活性的影响,通过定位施肥试验,于春茶、夏茶、秋茶和冬管期,采集施用菌渣、生物炭+复合肥、有机肥、生物炭有机肥和复合肥(对照)为基肥,尿素为追肥的茶园土壤,研究不同肥料配施对重庆茶园土壤硝酸还原酶、亚硝酸还原酶、脲酶、N-乙酰-β-D-氨基葡萄糖苷酶和L-亮氨酸氨基肽酶5种氮转化酶活性的影响.结果表明:土壤氮转化酶活性随茶叶不同生产期表现出明显的动态变化,土壤硝酸还原酶、亚硝酸还原酶、脲酶和N-乙酰-β-D-氨基葡萄糖苷酶活性以春茶期最高,夏茶期最低.不同肥料配施尿素显著改变土壤氮转化酶活性.与对照相比,有机肥配施尿素对春茶期、夏茶期和秋茶期硝酸还原酶活性提升效果最佳,提升幅度达4.83%、4.49%和7.87%;菌渣配施尿素对土壤亚硝酸还原酶、脲酶和N-乙酰-β-D-氨基葡萄糖苷酶活性促进最大,分别较对照增加32.48%、46.43%和75.24%;不同基肥配施尿素抑制了土壤L-亮氨酸氨基肽酶活性.冗余分析显示,微生物生物量氮是影响土壤氮转化酶活性的主导因子.因此,重庆地区茶园追肥施用尿素时,基肥选用菌渣有利于维持土壤氮转化酶活性.(图7表2参40)
To reveal the effects of different fertilizers and urea, their combined application on nitrogen transformation enzyme activities in tea-garden soil was examined. Locational fertilization tests were conducted to assess the effects of different fertilization regimes on the activities of nitrate reductase, nitrite reductase, urease, N-acetyl-β-D-glucosaminidase, and L-leucine aminopeptide in tea-garden soil from Chongqing. The experiment consisted of five different base fertilizer treatments, including mushroom residue, biological carbon combined with compound fertilizer, organic fertilizer, biochar organic fertilizer and compound fertilizer(CK), and used urea as a topdressing fertilizer. Tea-garden soils were sampled during the spring, summer, autumn, and winter tea production periods. The results indicate that soil nitrogen transformation enzyme activities are dynamic during different periods of tea production. The highest and lowest values of soil nitrate reductase, nitrite reductase, urease, and N-acetyl-β-D-glucosaminidase were observed in spring and summer tea production periods, respectively. The combined application of different fertilizers and urea significantly affected nitrogen transformation enzyme activities. Compared to CK, the organic fertilizer and urea combined application had the greatest effect on the improvement of nitrate reductase activity in spring, summer, and autumn tea production periods, with an increase of 4.83%, 4.49%, and 7.87%, respectively;mushroom residue and urea combined application had the greatest effect on the improvement of soil nitrite reductase, urease, and N-acetyl-β-Dglucosidase activity, with an increase of 32.48%, 46.43%, and 75.24%, respectively;different base fertilizers and urea combined application inhibited soil L-leucine aminopeptide activity. The redundancy analysis suggested that soil microbial biomass nitrogen was the dominant factor affecting the activities of soil nitrogen transformation enzymes. When urea is applied as fertilizer in tea gardens in Chongqing
作者
杨海滨
李中林
邓敏
盛忠雷
张莹
胡方洁
邬秀宏
徐泽
YANG Haibin;LI Zhonglin;DENG Min;SHENG Zhonglei;ZHANG Ying;HU Fangjie;WU Xiuhong;XU ze(Tea Research Institute of Chongqing Academy of Agricultural Sciences,Yongchuan 402160,China)
出处
《应用与环境生物学报》
CAS
CSCD
北大核心
2020年第5期1107-1114,共8页
Chinese Journal of Applied and Environmental Biology
基金
国家重点研发计划项目(2016YFD0200906)
重庆市社会民生重点研发项目(cstc2017shms-zdyf X0011)
一般项目(cstc2018jscxmsybX0237)资助。
关键词
肥料配施
尿素
氮转化酶
茶园土
fertilization regime
urea
nitrogen transformation enzyme
tea-garden soil
