摘要
目的:探讨稀有细胞群单细胞分选方案的优化。方法:分析仪器设备的设置、分选前细胞的准备和分选方案的选择。结果:通过提供的单细胞分选的方案,使单克隆形成率达86.5%。结论:建立了单细胞分选的优化方案,并重点分析了提高单克隆形成率的影响因素和解决方法。
Objective:To explore the optimization of single cell sorting in rare cell populations.Methods:The setup of instrument and equipment,the preparation of cells before sorting and the selection of sorting scheme were analyzed.Results:Using the single cell sorting scheme provided in this paper,the monoclonal formation rate reached 86.5%.Conclusion:In this paper,the optimization scheme of single cell sorting was established,and the influencing factors and solutions to improve the monoclonal formation rate are analyzed emphatically.
作者
宋兴辉
李艳伟
王佳佳
邢月婷
黄莹莹
郭春
Song Xing-hui;Li Yan-wei;Wang Jia-jia;Xing Yue-ting;Huang Ying-ying;Guo Chun(Zhejiang University,Zhejiang Hangzhou,310058)
出处
《生物化工》
2020年第4期92-93,96,共3页
Biological Chemical Engineering
基金
浙江大学实验技术研究项目(SJS201712,SJS201714)
浙江省基础公益研究计划项目(LGF19H150006)
浙江省教育厅一般科研项目(Y201941524)。
关键词
单细胞分选
单克隆形成率
细胞活性
single cell sorting
monoclonal formation rate
cell activity
