摘要
通过测量菌丝直径和干重对松乳菇(rcb-74)和靓丽乳菇(rll-107,rmsh-118)进行最适菌丝生长培养基的筛选。结果表明所有菌株均在改良的biotin-aneurine-folic acid(BAF)培养基上表现出较大的菌丝直径以及最大菌丝干重。用改良的BAF培养基制备乳菇液体菌丝,接种云南松、马尾松和油松,并在13–30d后发现rcb-74与云南松和油松形成菌根,rll-107与马尾松形成菌根,rmsh-118与云南松形成菌根。
Optimum culture medium for Lactarius deliciosus rcb-74,L.vividus rll-107 and L.vividus rmsh-118 was screened by measuring diameter and dry weight of mycelia.The results showed that all isolates had relatively large diameter of mycelia and the maximum dry weight on modified biotin-aneurine-folic acid(BAF)medium.Lactarius mycelium cultured in modified BAF liquid medium was inoculated to Pinus yunnanensis,P.massoniana and P.tabuliformis,and mycorrhizae were observed in combinations of L.deliciosus rcb-74 with P.yunnanensis and P.tabuliformis,combination of L.vividus rll-107 with P.massoniana,as well as combination of L.vividus rmsh-118 with P.yunnanensis in 13–30 days.
作者
王冉
Guerin-Laguette Alexis
于富强
WANG Ran;Guerin-Laguette Alexis;YU Fu-Qiang(CAS Key Laboratory for East Asia Biodiversity and Biogeography,Kunming Institute of Botany,Chinese Academy of Sciences,Kunming,Yunnan 650201,China;SWFU-KIB CAS Joint Institute for Applied Mycology,Kunming,Yunnan 650224,China;The New Zealand Institute of Plant and Food Research Limited,Christchurch 8053,New Zealand)
出处
《菌物学报》
CAS
CSCD
北大核心
2020年第7期1346-1355,共10页
Mycosystema
基金
国家自然科学基金(31901204)
中国科学院昆明植物研究所引进人才项目(Y9627111K1)。
关键词
乳菇
培养基
菌根合成
Lactarius
media
mycorrhizal synthesis
