摘要
目的:探讨木犀草苷对皮肤鳞状细胞癌细胞增殖、迁移和侵袭的影响及其机制。方法:人皮肤鳞状细胞癌细胞A431购自美国标准生物品收藏中心。分别以80 μmol/L(木犀草苷-低组)、160 μmol/L(木犀草苷-中组)和240 μmol/L(木犀草苷-高组)木犀草苷处理A431人皮肤鳞状细胞癌细胞株,对照组未行木犀草苷处理,实时定量反转录聚合酶链反应(RT-qPCR)检测细胞中肿瘤抑癌基因7-L(ST7L)基因信使核糖核酸(mRNA)的表达,蛋白质印迹法(Western blot)检测ST7L、p21、周期蛋白D1(Cyclin D1)、基质金属蛋白酶-2(MMP-2)、基质金属蛋白酶-9(MMP-9)等基因蛋白表达水平,细胞计数试剂盒(CCK-8)法测定A431细胞增殖抑制率,Transwell实验检测细胞迁移和侵袭能力。多组间比较采用单因素方差分析(组间比较采用SNK- q检验)。 结果:木犀草苷-低组、中组、高组A431细胞的增殖抑制率上升[(10.22±1.02)%、(23.51±2.36)%、(49.28±4.33)%, F=644.968, P<0.05],迁移[(87.36±8.33)%、(71.02±7.05)%、(55.32±5.43)%, F=81.657, P<0.05]和侵袭细胞数[(73.65±7.22)个、(60.25±6.03)个、(41.25±4.33)个, F=105.695, P<0.05]均降低,细胞中Cyclin D1(0.50±0.05、0.38±0.04、0.24±0.03, F=116.198, P<0.05)、MMP-2(0.57±0.05、0.44±0.04、0.31±0.03, F=107.242, P<0.05)和MMP-9(0.43±0.04、0.31±0.03、0.19±0.02, F=194.667, P<0.05)基因蛋白含量降低,p21(0.46±0.04、0.61±0.06、0.76±0.00, F=117.900, P<0.05)和ST7L(1.61±0.15、2.11±0.21、2.84±0.27, F=118.059, P<0.05)基因蛋白的含量升高。过表达ST7L基因可提高A431细胞的增殖抑制率[(40.56±4.32)%]和p21蛋白(0.73±0.06)含量,降低迁移[(61.58±6.17)%]和侵袭[(52.65±5.23)个]细胞数,下调细胞中Cyclin D1(0.29±0.03)、MMP-2(0.36±0.03)和MMP-9(0.24±0.03)基因蛋白表达,差异均有统计学意义( P<0.05)。抑制ST7L基因表达可逆转木犀草苷对A431细胞增殖、迁移和侵袭的作用。 结论:木犀草苷可抑制皮肤鳞状细胞癌细胞增殖、�
Objective To investigate the effects of luteoloside on proliferation,migration and invasion of skin squamous carcinoma cells and the mechanism.Methods A431 cells(From American type culture collection)were treated with luteoloside at 80μmol/L(low-luteoloside group),160μmol/L(median-luteoloside group)and 240μmol/L(high-luteoloside group).The levels of ST7L mRNA in A431 cells was detected by real-time quantitative reverse transcriptase-polymerase chain reaction(RT-qPCR).The expression levels of ST7L,p21,Cyclin D1,matrix metalloproteinase(MMP)-2 and MMP-9 proteins were detected by Western blotting.The proliferation inhibition rate of A431 cells was determined by cell counting kit-8(CCK-8)assay,and the migration and invasion abilities of A431 cells were measured by Transwell assay.Results In the low-luteoloside group,median-luteoloside group and high-luteoloside group,the proliferation inhibition rate of A431 cells were increased[(10.22±1.02)%,(23.51±2.36)%,(49.28±4.33)%,F=644.968,P<0.05],the number of migrating cells[(87.36±8.33)%,(71.02±7.05)%,(55.32±5.43)%,F=81.657,P<0.05]and invading cells(73.65±7.22,60.25±6.03,41.25±4.33,F=105.695,P<0.05)was decreased,the contents of Cyclin D1(0.50±0.05,0.38±0.04,0.24±0.03,F=116.198,P<0.05),MMP-2(0.57±0.05,0.44±0.04,0.31±0.03,F=107.242,P<0.05)and MMP-9(0.43±0.04,0.31±0.03,0.19±0.02,F=194.667,P<0.05)proteins were down-regulated,and the contents of p21(0.46±0.04,0.61±0.06,0.76±0.0,F=117.900,P<0.05)and ST7L(1.61±0.15,2.11±0.21,2.84±0.27,F=118.059,P<0.05)were increased.In A431 cells,over-expression of ST7L increased the inhibition rate of proliferation(40.56±4.32)of A431 cells,reduced the number of migrating cells(61.58±6.17)and invading(52.65±5.23),and down-regulated the levels of Cyclin D1(0.29±0.03),MMP-2(0.36±0.03)and MMP-9(0.24±0.03)proteins.Inhibition of ST7L reversed the effects of luteoloside on proliferation,migration and invasion of A431 cells.Conclusion Luteoloside inhibits proliferation,migration and invasion of A431 cells by promoting the
作者
沈琴
王攀
黄长征
张万里
卢静静
Shen Qin;Wang Pan;Huang Changzheng;Zhang Wanli;Lu Jingjing(Department of Dermatology,Hubei Provincial Hospital of Traditional Chinese Medicine,Wuhan 430074,China;Department of Dermatology,Wuhan Children’s Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430014,China;Department of Dermatology,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China;Department of Cancer Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430022,China)
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2020年第5期914-917,共4页
Chinese Journal of Experimental Surgery
基金
国家自然科学基金(81972565)
武汉市卫生计生委科研计划资助项目(WX18Q45)。
关键词
皮肤鳞状细胞癌
木犀草苷
肿瘤抑癌基因7-L
机制
Skin squamous cell carcinoma
Luteoloside
Suppression of tumorigenicity 7 like
Mechanism
