摘要
目的探讨微小RNA(miR)-561在食管鳞状细胞癌中的临床意义及其表达。方法选取2015年1月至2016年2月在湖北医药学院附属东风医院行手术治疗的食管鳞癌患者癌旁组织及癌组织标本96例,检测miR-561的表达水平;细胞培养实验检测Het-1a、Kyse150及Eca109等细胞株中miR-561表达水平;统计食管鳞癌的临床特征与miR-561表达水平的相关性;分析食管鳞癌患者miR-561的表达水平与预后的关系。结果食管鳞癌细胞株Eca109、Kyse150中miR-561相对表达量显著低于细胞株Het-1a(1.61±0.30、1.21±0.28比2.56±0.51),差异有统计学意义(P<0.05)。食管鳞癌患者癌组织中的miR-561相对表达量明显低于癌旁组织中的表达量(0.80±0.17比1.51±0.42),差异有统计学意义(P<0.05)。有无饮酒史、淋巴结转移、分化程度及肿瘤分期均与食管鳞癌组织中miR-561表达水平有相关性(P<0.05)。Cox多因素回归分析结果显示,影响患者预后的独立危险因素为miR-561表达、TNM分期及淋巴结转移(P<0.05)。miR-561表达未下降食管鳞癌患者随访3生存率明显高于miR-561表达下降患者,差异有统计学意义(P<0.05)。结论在食管鳞状细胞癌患者中miR-561存在低水平表达,而且食管鳞癌的发展、发生及预后都与其关系密切。
Objective To investigate the clinical significance and expression of microRNA(miR)-561 in esophageal squamous cell carcinoma.Methods From January 2015 to February 2016,96 specimens of adjacent tissues and cancer tissues from patients with esophageal squamous cell carcinoma who underwent surgery in Dongfeng Hospital Affiliated of Hubei University of Medicine were selected to detect the expression level of miR-561.Cell culture experiments were used to detect the expression level of miR-561 in Het-1a,Kyse150 and Eca109 cell lines.The correlation between clinical features and the expression level of miR-561 was counted.The expression level of miR-561 in patients with esophageal squamous cell carcinoma was analyzed.The relationship between the level of miR-561 and prognosis was investigated.Results Relative expression of miR-561 in esophageal squamous cell carcinoma cell lines Eca109 and Kyse150(1.61±0.30,1.21±0.28)was significantly lower than that in Het-1a(2.56±0.51),and the difference was statistically significant(P<0.05).The relative expression of miR-561 in esophageal squamous cell carcinoma tissues was significantly lower than that in adjacent tissues(0.80±0.17 vs.1.51±0.42),and the difference was statistically significant(P<0.05).The expression of miR-561 in esophageal squamous cell carcinoma was correlated with alcohol drinking history,lymph node metastasis,differentiation degree and tumor stage(P<0.05).Cox multivariate regression analysis showed that the independent risk factors affecting the prognosis of patients were the expression of miR-561,TNM stage and lymph node metastasis(P<0.05).The survival rate of patients with esophageal squamous cell carcinoma whose expression of miR-561 did not decrease at follow-up was significantly higher than that of patients with decreased expression of miR-561(P<0.05).Conclusions In patients with esophageal squamous cell carcinoma,the expression of miR-561 is low,and the development,occurrence and prognosis of esophageal squamous cell carcinoma are closely related with
作者
赵铮
李毅
杨飞翔
Zhao Zheng;Li Yi;Yang Feixiang(Department of Clinical Laboratory,Dongfeng Hospital Affiliated of Hubei University of Medicine,Hubei Shiyan 442000,China)
出处
《中国医师进修杂志》
2019年第12期1107-1111,共5页
Chinese Journal of Postgraduates of Medicine
