摘要
通过室内土柱模拟实验,采用多种土壤指标测定法和高通量测序手段探究了再生水回灌过程中再生水对土壤理化性质和土壤细菌的影响.结果表明,经再生水淋滤,9个样品土壤腐殖酸(HA)平均含量减少0.59 g·kg^-1,土壤pH值增加0.08,有机碳(SOC)、重金属铜(Cu^2+)平均含量分别增加了2.02 g·kg^-1,0.923 g·kg^-1.土壤原始样品经高通量测序占优势的菌种有变形菌门(Proteobacteria 27.38%)、放线菌门(Actinobacteria 24.68%)等,淋滤后变形菌门丰度升高至37.27%,放线菌门减少至20.62%,说明再生水淋滤后土壤环境发生较大变化.
The recharge of reclaimed water to recharge groundwater is an effective measure to alleviate the lack of groundwater resources in the region.However,the research on the effects of reclaimed water on soil physical and chemical properties and soil bacteria in the process of recharge is limited.In this study,through indoor soil column simulation experiments,a variety of soil index determination methods and high-throughput sequencing methods were used to explore the effects of reclaimed water on soil physical and chemical properties and soil bacteria during reclaimed water recharge.Studies showed that the average content of humus in nine samples is reduced by 0.59 g·kg^-1,the soil pH,organic carbon(SOC),and heavy metal copper(Cu^2+)increased by 0.08,2.02 g·kg^-1,and 0.923 g·kg^-1 respectively after leaching with reclaimed water.The strains dominated by high-throughput sequencing of soil samples include Proteobacteria(27.38%)and Actinobacteria(24.68%),after leaching,the abundance of Proteobacteria increased to 37.27%,and Actinobacteria decreased to 20.62%.The leaching of reclaimed water had a great influence on the composition of soil bacterial community,and the proportion of Proteobacteria increased,indicating that the soil environment changed greatly after leaching of reclaimed water.Compared with the existing results,this study supplements the research on the effects of reclaimed water reuse on soil physical and chemical properties and soil bacteria,and provides a new research idea and method for the influence of reclaimed water on soil environment.At the same time,it provides a parameter basis for groundwater recharge and land subsidence treatment,and achieves the purpose of protecting the underground environment.
作者
高远
林山杉
李天宇
胡正
GAO Yuan;LIN Shan-shan;LI Tian-yu;HU Zheng(School of Environment,Northeast Normal University,Changchun 130117,Jilin,China)
出处
《西北师范大学学报(自然科学版)》
CAS
北大核心
2019年第4期100-106,共7页
Journal of Northwest Normal University(Natural Science)
基金
国家自然科学基金资助项目(41772236)
长春市科技局重大科技攻关项目(17SS027)
关键词
再生水
回灌
理化性质
土壤细菌
高通量测序
reclaimed water
recharge
physical and chemical properties
soil bacteria
high-throughput sequencing