摘要
目的探讨复方尿囊素对感染幽门螺杆菌(H.pylori)的昆明鼠疗效及协同性评价。方法健康雄性昆明鼠(每组12只),随机数字表法分7组。分别为正常对照组、H.pylori感染对照组、复方尿囊素组、尿囊素组、氢氧化铝组、三联治疗(克拉霉素+阿莫西林+雷贝拉唑)组以及复方尿囊素联合三联治疗组(联合组)。正常对照组行生理盐水间隔1 d灌胃,连续灌胃5次,其他组行同周期H.pylori[1×10^9 CFU悉尼菌株(SS1)]灌胃5次,造模4周后,用上述各组药物连续6 d灌胃干预,1 d后按动物伦理处死动物,快速尿素酶试验(RUT)和免疫组化检测各组H.pylori感染情况,光镜及电镜下观察各组病理及超微病理损伤情况。结果RUT和免疫组化两种方法检测H.pylori感染状态,复方尿囊素组的阳性率9.1%和0,显著低于H.pylori感染对照组(81.8%比72.7%,均P<0.05)。尿囊素组(27.3%和18.2%)与氢氧化铝组(54.5%和54.5%)阳性率差异均无统计学意义(均P>0.05)。其中,氢氧化铝组阳性率显著高于复方尿囊素组(P<0.05)。联合组两种检测方法阳性率均为0,显著低于三联组阳性率(36.4%、45.5%)(均P<0.05),三联组与复方尿囊素组比较,差异均无统计学意义(均P>0.05)。复方尿囊素组病理及超微病理损伤较H.pylori感染对照组明显减轻。结论复方尿囊素对小鼠H.pylori感染具有治疗作用,与三联方案联合应用,可以进一步增强其H.pylori治疗作用。复方尿囊素对H.pylori所致的胃黏膜损伤有修复作用,其胃黏膜修复作用可能与线粒体途径有关。
Objective To investigate the therapeutic effect of compound allantoin in Kunming mice infected with Helicobacter pylori (H. pylori). Methods Eighty four male Kunming mice were randomly divided into normal control group, H. pylori infection control group, compound allantoin group, allantoin group, aluminum hydroxide group, triple therapy group, and compound allantoin combined with triple therapy group(drug combination group). The normal control group was administered with normal saline, and other groups were infected with H. pylori for 5 times by intragastric(IG) administration. After 4 weeks, mice were given corresponding drug solutions for 6 times by IG administration. H. pylori infection status was detected by rapid urease test(RUT) and immunohistochemistry assay(IHC). Mucosa damages were assessed by microscopic examination and electron microscopy. Results The positive rates of the compound allantoin group detected by RUT and IHC were 9.1% and 0, respectively, which were significantly lower than those in the H. pylori infection control group (81.8% and 72.7%).The positive rates of aluminum hydroxide group(54.5% and 54.5%) have no significant difference with those in the allantoin group (27.3% and 18.2%), but were higher than those of compound allantoin group (P<0.05).The positive rate of both methods in the drug combination group were 0, and they were significantly lower than those in the triple therapy group (36.4%,45.5%)(P<0.05). There was no difference between the triple group and the compound allantoin group(P>0.05). The pathological and ultrastructural damage of compound allantoin group was obviously relieved than that of H. pylori infection group. Conclusion Compound allantoin has therapeutic effect on H. pylori infection in mice, which can be further enhanced by combination with triple therapy group. In addition, compound allantoin can repair gastric mucosal injury caused by H. pylori, and its repair effect may be related to mitochondrial pathway.
作者
杨悦
李德善
胡伏莲
李超
杨桂彬
Yang Yue;Li Deshan;Hu Fulian;Li Chao;Yang Guibin(Department of Gastroenterology, Peking University Aerospace School of Clinical Medicine, Aerospace Center Hospital, Beijing 100049, China;Department of General Medicine, Peking University Aerospace School of Clinical Medicine, Aerospace Center Hospital, Beijing 100049, China;Department of Gastroenterology, First Hospital of Peking University, Beijing 100034, China)
出处
《中华医学杂志》
CAS
CSCD
北大核心
2019年第20期1541-1545,共5页
National Medical Journal of China
关键词
幽门螺杆菌
复方尿囊素
昆明鼠
Helicobacter pylori
Compound allantoin
Kunming mice
