摘要
研究青钱柳多糖(CPP)和青钱柳黄酮(CPF)对胰岛素抵抗的人源肝癌细胞(IR-HepG2)葡萄糖消耗量及α-葡萄糖苷酶活性影响。用高浓度胰岛素诱导培养HepG2细胞,建立IRHepG2细胞模型,将IR-HepG2细胞分为模型组、CPP高、中、低剂量组、CPF高、中、低剂量组和二甲双胍组,各组以相应药物孵育24 h,采用葡萄糖试剂盒测定细胞葡萄糖消耗量(△GC),MTT法测定单位细胞葡萄糖消耗量(△GC/OD);以阿卡波糖为阳性对照,比较不同浓度梯度的CPP与CPF对α-葡萄糖苷酶活性影响。与模型组比较,阴性对照组、二甲双胍组、CPP组与CPF组细胞△GC与△GC/OD显著性升高;不同浓度的CPP与CPF对α-葡萄糖苷酶均有抑制作用。提示青钱柳活性提取物降血糖功效与其提高细胞葡萄糖摄取量,抑制α-葡萄糖苷酶活性有关。
The effects of Cyclocarya paliurus(Batal.) Iljinskaja Extracts on glucose consumption of insulin resistance HepG2 cell and activity of alpha-glucosidase is studied. HepG2 cell with high concentration insulin(10 μg/mL) is induced to establish insulin resistance cell model and divided model cell into Cyclocarya paliurus polysaccharide(CPP),Cyclocarya paliurus flavone(CPF) and dimethylbiguanide groups. After 24 h administration,glucose assay is used fit to measure the gross glucose consumption of cells(△GC), glucose consumption of unite cell(△GC/OD) is detected by MTT assay. The inhibitory activity on alpha-glucosidase of CPP and CPF is evaluated,compared with acarbose,in vitro. Compared with model group,△GC and △GC/OD of IR-HepG2 cell is increased by CPF and CPP significantly. CPP and CPF have inhibitory effect on the activity of alpha-glucosidase. Those results indicat that CPF and CPP have effects on increasing the glucose consumption of cell and decreasing the activity of alpha-glucosidase,thus alleviating hyperglycemia.
作者
王胤康
吕萌
许琦
陈伟鸿
谭开祥
向极钎
刘卫
WANG Yinkang;LYU Meng;XU Qi;CHEN Weihong;TAN Kaixiang;XIANG Jiqian;LIU Wei(College of Life Science and Technology,Huazhong University of Science and Technology,Wuhan 430074,China;Wuhan Best-Carrier Nanotechnology Co. Ltd,Wuhan 430075,China;Infinitus Co. Ltd,Guangzhou 510665,China;Sihui Biotechnology Co. Ltd,Enshi 445099,China;Enshi Academy of Agricultural Science,Enshi 445099,China)
出处
《食品与生物技术学报》
CAS
CSCD
北大核心
2019年第2期120-125,共6页
Journal of Food Science and Biotechnology
基金
湖北省科技支撑计划项目(2013BEC048)
关键词
青钱柳
HEPG2细胞
多糖
黄酮
Α-葡萄糖苷酶
Cyclocarya paliurus(Batal.) Iljinskaja
HepG2 cell
polysaccharide
flavone
alpha-glucosidase
