摘要
【目的】分析低温(4℃)胁迫下甘蓝(Brassica oleracea L.)幼叶中还原型抗坏血酸—还原型谷胱甘肽(AsAGSH)循环代谢的变化,为选育和推广抗寒甘蓝品种提供参考依据。【方法】以4个不同耐寒性甘蓝品系为试验材料,测定分析低温胁迫下其过氧化氢(H2O2)、丙二醛(MDA)、AsA、GSH、氧化型谷胱甘肽(GSSG)、氧化型抗坏血酸(DHA)含量和AsA/DHA、GSH/GSSG及抗坏血酸过氧化物酶(APX)、谷胱甘肽还原酶(GR)和单脱氢抗坏血酸还原酶(MDAR)活性的变化情况。【结果】随低温胁迫时间的延长,4个甘蓝品系幼叶的H2O2和MDA含量均明显增加;耐寒甘蓝品系231和235的AsA、GSH和GSSG含量显著增加(P<0.05,下同),不耐寒甘蓝品系161和163的AsA、GSH和GSSG含量则呈先增加后减少的变化趋势;DHA含量均呈增加趋势,AsA/DHA和GSH/GSSG均呈先增加后减少的变化趋势,但不同耐寒性甘蓝品系间存在差异;AsA-GSH循环中的APX、GR和MDAR活性在低温胁迫期间均呈上升趋势,而脱氢抗坏血酸还原酶(DHAR)活性呈先升高后降低的变化趋势。【结论】耐寒甘蓝品系231和235能更迅速响应低温胁迫,可有效清除H2O2,减轻氧化应激损伤,维持甘蓝体内的氧化还原平衡。
【Objective】This study was undertaken to investigate the effects of low temperature stress(4℃)on the reduced ascorbic acid-glutathione(AsA-GSH)cycle metabolism in leaves of cabbage seedlings and provide reference for breeding and improving cold resistant cabbage varieties.【Method】Under low temperature stress,variations of hydrogen peroxide(H2O2)content,malondialdehyde(MDA)content,AsA content,GSH content,glutathione disulfide(GSSG)content,dehydroascorbic acid(DHA)content,AsA/GSH ratio,GSH/GSSG ratio,ascorbate peroxidase(APX)activity,glutathione reductase(GR)activity,monodehydroascorbate reductase(MDAR)activity were determined on four cold resistant cabbage lines.【Result】As low temperature stress time expanded,H2O2 and MDA contents in leaves of four cabbage lines increased largely,the contents of AsA,GSH and GSSG in cold-resistant cabbage lines(231 and 235)showed a significant increasing trend(P<0.05,the same below).While the contents of AsA,GSH and GSSG in cold-sensitive lines(161 and 163)increased first,and then decreased.The DHA content in the four lines increased.The AsA/GSH ratio and GSH/GSSG ratio increased first,and then decreased,but difference existed between different cold resistant lines.Activities of APX,GR and MDAR presented increasing trend in AsA-GSH cycle under low temperature stress,but dehydroascorbate reductase(DHAR)activity increased first,and then reduced.【Conclusion】Cold resistant lines 231 and 235 can respond to low temperature stress quickly,eliminate H2O2 effectively,mitigate oxidative injury,and maintain redox equilibrium in cabbage.
作者
山溪
秦文斌
张振超
姚悦梅
肖燕
戴忠良
SHAN Xi;QIN Wen-bin;ZHANG Zhen-chao;YAO Yue-mei;XIAO Yan;DAI Zhong-liang(Zhenjiang Institute of Agricultural Sciences in Hilly Area,Jurong,Jiangsu 212400,China)
出处
《南方农业学报》
CAS
CSCD
北大核心
2018年第11期2230-2235,共6页
Journal of Southern Agriculture
基金
江苏省重点研发计划项目(BE2018423)
江苏省自主创新项目[cx(17)2019]
关键词
甘蓝
低温胁迫
AsA-GSH循环
生理生化指标
cabbage
low temperature stress
AsA-GSH cycle
physiological and biochemical index
