摘要
采用目标起始密码子多态性(SCo T)分子标记对17个枸杞品种进行遗传多样性分析。从80条引物中筛选出19条重复性好、条带清晰的引物进行PCR扩增,共扩增到96个条带,其中多态性条带71条,多态性比率为73. 96%。多态信息含量(PIC)变化范围为0. 055(SCo T-29)~0. 239(SCo T-66),平均为0. 132。聚类分析和主坐标分析结果表明,供试的17个枸杞品种遗传相似系数为0. 43~1. 00,在遗传相似系数为0. 87处可将所有品种分为3个类群。综上,利用SCo T分子标记可揭示枸杞品种资源的遗传多样性,为枸杞品种资源保护和利用提供科学依据。
Genetic diversity of 17 goji(wolfberry)cultivars was analyzed by start codon targeted polymorphism(SCoT)molecular marker.Nineteen SCoT primers generating clear and reproducible bands were obtained through screening a total of eighty primers.A total of 96 bands were amplified,of which 71(73.96%)were polymorphic.The value of polymorphism information content(PIC)varied from 0.055(SCoT-29)to 0.239(SCoT-66),with average of 0.132.Cluster analysis and principal coordinated analysis indicated that the genetic similarity(GS)varied from 0.43 to 1.00.All cultivars could be divided into three groups at the genetic similarity of 0.87.So SCoT markers could be effectively used to evaluate the genetic diversity of goji(wolfberry)cultivars germplasms.These results would provide references for protection and rational utilization of wolfberry cultivars.
作者
马利奋
尹跃
赵建华
王亚军
戴国礼
安巍
MA Lifen;YIN Yue;ZHAO Jianhua;Wang Yajun;DAI Guoli;AN Wei(Wolfberry Industry Development Centre of Ningxia,Yinchuan 750001,China;National Wolfberry Engineering Research Center,Ningxia Academy of Agriculture and Forestry Sciences,Yinchuan 750002,China)
出处
《浙江农业学报》
CSCD
北大核心
2018年第10期1665-1670,共6页
Acta Agriculturae Zhejiangensis
基金
宁夏农业特色优势产业新品种选育专项(2013NYYZ0101)
关键词
枸杞
品种
SCoT标记
遗传多样性
goji(wolfberry)
cultivar
SCoT marker
genetic diversity
