摘要
目的:探究PET材料人工韧带表面羟基磷灰石(HAp)/明胶(Gel)涂层对移植物-宿主骨愈合的影响。方法:按PET材料人工韧带表面涂层情况分为2组:PET组和PET/HAp/Gel涂层组。用扫描电镜分别观察两种材料表面涂层情况。对48只雄性新西兰大白兔行前交叉韧带重建术,将两种材料分别植入,并于术后4周、8周处死取材,进行组织学HE染色观察和生物力学检测。结果:扫描电镜观察显示涂层覆载成功。组织学HE染色观察显示,术后4周移植物-宿主骨界面形成较厚的纤维结缔组织,术后8周两组界面宽度均缩小,PET/HAp/Gel涂层组界面宽度缩小幅度较大,且于术后8周发现PET/HAp/Gel涂层组界面中有新生骨形成。生物力学检测显示,术后8周时,PET组与PET/HAp/Gel涂层组的断裂载荷(46.16±2.88 N vs 71.32±3.92 N)之间有显著统计学差异(P=0.0021);术后4周和8周,PET组与PET/HAp/Gel涂层组的刚度之间均有显著的统计学差异(11.06±1.14 N/mm vs 16.20±1.17 N/mm,P=0.0199;14.37±0.88 N/mm vs 24.35±1.35 N/mm,P=0.0008)。结论:HAp/Gel涂层材料能提升PET人工韧带的骨诱导性,促进移植物-宿主骨界面新生骨的形成,加强移植物-宿主骨愈合。
Ojective To explore the effect of the hydroxyapatite（HAp）and gelatin（Gel）coating on thehealing of the polyethylene terephthalate（PET）artificial ligament. Methods The artificial ligaments weredivided into a PET group with a pure PET surface and a PET/HAp/Gel group coated with HAp andGel. Both coatings were observed using the scanning electron microscope（SEM）. Forty-eight male NewZealand rabbits were randomly divided into two groups and underwent anterior cruciate ligament recon-struction, before two kinds of artificial ligaments were implanted respectively. Four weeks and 8 weeksafter the operation, the rabbits were sacrificed, and histological hematoxylin and eosin（HE）staining aswell as the biomechanical examination were performed. Results HAp/Gel coating was found depositingon the surface of PET artificial ligaments. Histological HE staining showed a thick fibrous connectivetissue forming at the graft-host bone interface 4 weeks postoperatively, and the interface width of bothgroups were narrowed, with significantly more shrinking in the PET/HAp/Gel coating group. And newbone tissues were found in the interface of PET/HAp/Gel group 8 weeks after the operation. The biomechanical examination found significant differences in the failure load between the PET（46.16 ± 2.88 N）and PET/HAp/Gel group（71.32 ± 3.92 N）8 weeks after the surgery（P=0.0021）. And 4 weeks and 8 weeks after the surgery, significant differences were found in the stiffness between the PET group andthe PET/HAp/Gel group（11.06 ± 1.14 N/mm vs 16.20 ± 1.17 N/mm, P=0.0199; 14.37 ± 0.88 N/mmvs 24.35 ± 1.35 N/mm, P=0.0008）. Conclusion HAp/Gel coating can enhance the osteogenesis of PETartificial ligaments, promoting the new bone formation at the graft-host bone interface and hereinstrengthening the graft-host bone healing.
作者
盛旦丹
艾承冲
蔡江瑜
万方
金文赫
汪斯衡
吕婧仪
智云龙
张鹏
陈俊
蒋佳
陈世益
Sheng Dandan;Ai Chengchong;Cai Jiangyu;Wan Fang;Jin Wenhe;Wang Siheng;Lv Jingyi;Zhi Yunlong;Zhang Peng;Chen Jun;Jiang Jia;Chen Shiyi(Department of Sports Medicine, Huashan Hospital, Fudan University, Shanghai 200040, Chin)
出处
《中国运动医学杂志》
CAS
CSCD
北大核心
2018年第5期400-405,共6页
Chinese Journal of Sports Medicine
基金
国家高技术研究发展计划(863计划)(课题编号:2015AA033703)
国家重点研发计划项目(编号:2016YFC1100300
2017YFC0840100和2017YFC0840106)
国家自然科学基金(编号:81572108和81772339)
上海市重中之重临床医学中心项目(编号:2017ZZ01006)
上海市启明星计划(编号:18QB1400500)
深圳三名工程(编号:SZSM20161207)
