摘要
目的:探讨携带HBV孕产妇血清免疫标志物(HBV-M)含量与乙型肝炎病毒DNA(HBVDNA)载量之间的关系。方法:应用电化学发光法(ECLIA)检测240例携带HBV孕产妇血清标本中HBsAg、HBsAb、HBeAg、HBeAb、HBcAb含量,用荧光定量聚合酶链式反应(FQ-PCR)技术同时检测HBV-DNA载量,对2组检测结果进行统计学分析。结果:ECLIA检出不常见模式47例,出现10种血清模式,模式1~5组均有HBV-DNA阳性检出,其中模式1,2,5组的检出率为100%,HBV-DNA载量分别为6E+07±9.02E+07、4.85E+06±3.01E+06、1.51E+07±2.13E+07,模式6~10组HBV-DNA载量<5E+02;HBV-DNA阳性患者HBsAg、HBsAb、HBeAg的定量结果与阴性患者差异有统计学意义(P<0.05),而HBV-DNA阳性患者HbeAb、HBcAb的定量结果与阴性患者差异无统计学意义(P>0.05)。240例携带HBV孕产妇血清HBsAg、HBeAg、HBeAb含量与HBV-DNA载量呈正相关(r=0.657、0.562、0.501,P<0.05),HBsAb含量与HBV-DNA载量呈负相关(r=-0.438,P<0.05)。结论:HBV-M含量和HBV-DNA载量密切相关,建议将两者相结合,合理制定HBV母婴传播的预防措施。
Objective:To investigate the correlation between the carrying capacity of immunological marker of HBV and HBVDNA in serum of HBV-carrying maternal.Methods:Test HBsAg、HBsAb、HBeAg、HBeAb and HBcAb in serum samples from 240 HBV-carrying maternal with ECLIA.Test HBV-DNA carrying capacity with FQ-PCR in the meanwhile.Analyze the correlation statistically.Results:There are 47 rare patterns from group ECLIA from which appears 10 patterns in serum.There are HBV-DNA positive phenotypes in group 1~5,in which group 1,2 and 5 have a 100%positive ratio.The HBV-DNA carrying capacity of group 1,2 and 5 is6 E+07±9.02 E+07、4.85 E+06±3.01 E+06 and 1.51 E+07±2.13 E+07 respectively.The content of HBsAg,HBsAb and HBeAg of HBV-DNA positive serum samples is statistically different from those of negative ones(P<0.05)while there is no difference between the content of HBeAb and HBcAb(P>0.05).The content of HBsAg,HBeAg and HBeAb is positively correlated with HBV-DNA(r=0.657,0.562,0.501,P<0.05)whereas the content of HBsAb is negatively correlated with HBV-DNA(r=-0.438,P<0.05).Conclusion:The content of HBV Marker(HBV-M)is closely correlated with HBV-DNA carrying capacity.We should combine ECLIA and FQ-PCR to draw the preventive measures effectively for both mothers and their newborns.
出处
《长治医学院学报》
2017年第6期405-408,共4页
Journal of Changzhi Medical College
基金
2015年度山西省卫生计生科研课题(2015162)
关键词
HBV
电化学发光法
聚合酶链式反应法
HBV
Electrochemiluminescence immunoassay(ECLIA)
Fluorescent quantitative polymerase chain reaction technique(FQ-PCR)
