摘要
水稻NRT1.1B是一个高氮利用率基因,在育种中有着重要的应用价值。为提高NRT1.1B基因的选择效率,根据野生型NRT1.1B与突变型nrt1.1b基因存在的单核苷酸差异,结合四引物扩增受阻突变体系PCR的技术原理开发基因功能标记。使用8份含NRT1.1B基因的常规籼稻、8份含nrt1.1b基因的常规粳稻及4份含NRT1.1B/nrt1.1b基因的F1材料对功能标记进行检测验证,结果表明,所开发的基因功能标记可准确区分NRT1.1B基因的纯合显性、纯合隐性和杂合基因型,其扩增带型与基因型完全吻合,是一种鉴定NRT1.1B基因的有效方法。该方法操作简便,且费用低廉,可广泛应用于水稻NRT1.1B基因的资源鉴定和分子标记辅助选择育种。利用NRT1.1B功能标记,对本课题组的籼粳杂交育种材料进行鉴定,筛选出了一批含高氮利用率NRT1.1B的材料,为进一步的育种利用奠定了基础。
Rice NRT1.1B is a high nitrogen-use efficiency gene, which has important application value in breeding. In order to improve the efficiency of selection for NRT1.1B gene in breeding, a functional marker was developed by tetra-primer amplification refractory mutation system PCR based on single nucleotide differences between wild NRT1.1B and mutation nrt1.1b. Eight conventional indica rice containing NRT1.1B, eight conventional japonica rice containing nrt1.1b and four F1 from indica-japonica hybrid rice containing NRT1.1B/nrt1.1b were detected by the functional marker. The results showed that three genotypes involving the homozygous genotype with NRT1.1B,the homozygous genotype with nrt1.1b and the heterozygous genotype could be clearly distinguished according to band types, and it was completely consistent with the result of genotype analysis. Consequently, it is an effective method for molecular identification of NRT1.1B gene. As a simple operation and low-cost method, the NRT1.1B gene functional marker could be widely used to identify rice resources with NRT1.1B gene and applied in molecular marker-assisted breeding. The NRT1.1B gene functional marker was employed to analyze the rice breeding materials of hybridization between indica and japonica from our team, and A number of materials with high nitrogen-use efficiency NRT1.1B were selected, to lay the ground for the further breeding application.
出处
《分子植物育种》
CAS
CSCD
北大核心
2016年第2期410-416,共7页
Molecular Plant Breeding
基金
贵州省科技厅重大专项([2012]6005
[2013]6023)
贵州省毕节市科技计划项目([2012]34-3)共同资助
关键词
水稻
高氮利用率
NRT1.1B基因
功能标记
开发与应用
Rice
High nitrogen-use efficiency
NRT1.1B gene
Functional marker
Development and application
