摘要
目的开展人类免疫缺陷病毒/人结核分枝杆菌（HIV/MTB）双重感染病例分子分型及变异重组和流行特征研究,分析其传染源、变异性及其在人群中的传播特点和地区分布特征,为HIV/MTB双重感染病例的管理与临床精准治疗提供理论依据。方法对2014—2015年采集到的江西、广东、云南3省部分HIV/MTB双重感染173例患者115份确证的HIV阳性样本和105份鉴定的MTB菌株,分别用巢氏PCR方法和24位点MIRU-VNTR基因分型方法进行基因分型。结果流行病学个案调查资料结果显示,HIV/MTB双重感染病例以男性为主,年龄以中青年（18～59岁）为主,职业以农民、无业、务工/商人为主,传播途径以性传播和肺部感染为主,其感染顺序为先感染HIV后感染MTB占95%。分子分型结果显示,HIV/MTB双重感染病例样本中115份HIV阳性样本成功扩增分型85份,共发现8种HIV亚型/重组型;HIV/MTB双重感染病例中105份MTB样本成功扩增分型100份,结核分枝杆菌分为5个基因群（Ⅰ群、Ⅱ群、Ⅲ群、Ⅳ群、Ⅴ群）,共95个基因型。结论我国南方3省HIV/MTB双重感染病例中HIV主要流行亚型为CRF01_AE和CRF07_BC重组型;MTB主要流行株以I群和Ⅱ群为主,与单纯感染HIV和单纯感染MTB的流行株类型一致。HIV与MTB主要流行亚型之间是否存在相互易感还有待于进一步研究。
Objective To surveillance the molecular genotypes and epidemiological characteristics of HIV and MTB of HIV/MTB co-infection patients,analyze the genetic variability,transmission characteristics and regional distributions,and to provide a scientific basis for management and accurate treatment of the HIV/MTB populations. Methods 173 HIV/MTB cases,including 115 HIV positive samples and 105 MTB samples from Jiangxi,Guangdong,Yunnan province,were applied for genotyping by nested-PCR and 24-locus MIRU-VNTR,2014-2015. Results Epidemiological surveys showed that in HIV/MTB populations studied,men,young and middle-ages′（18-59） farmers,unemployed people and workers occupied the most;the prevalent transmission were sex and pulmonary infection. 95% were first infected with HIV. Molecular epidemiology study showed that 85 out of 115 HIV samples could be amplified successfully,with 8 different subtypes had been identified. For MTB,100 out of 105 samples could be amplified,with five groups（Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ）,95 genotypes.Conclusion The dominant HIV subtypes were CRF01_AE and CRF07_BC,and the dominant MTB genotypes were I andⅡin HIV/MTB populations,which were consistent with the HIV or MTB mono-infected populations. It is still need to study the interaction between the dominant subtypes of HIV and MTB.
作者
刘开明
赵明惠
马琳
刘阳
冉鑫
陈绛青
蔡卫平
杨欣平
万康林
覃卫红
LIU Kai-ming ZHAO Ming-hui MA Lin LIU Yang RAN Xin CHEN Jiang-qing CAI Wei-ping YANG Xin-ping WAN Kang-lin QIN Wei-hong(Jiangxi International Travel Healthcare Center, Nanchang, Jiangxi 330002, Chin)
出处
《中国国境卫生检疫杂志》
CAS
2016年第5期305-309,323,共6页
Chinese Journal of Frontier Health and Quarantine
基金
国家质检总局科技计划项目(2014IK0571)
