摘要
目的对比利巴韦林,观察宿主因子CaMKⅡ和PACT对甲型流感病毒RNA聚合酶的作用。方法采用双荧光素酶报告基因法检测293T细胞中甲型流感病毒聚合酶的相对表达量,分别判断利巴韦林处理与两种蛋白的沉默对甲型流感病毒RNA聚合酶的影响。结果沉默CaMKⅡ蛋白后流感病毒RNA聚合酶相对表达量降低,屏蔽PACT蛋白后甲型流感病毒RNA聚合酶相对表达量降低明显,但利巴韦林的处理对RNA聚合酶的抑制更为明显。结论利巴韦林能明显降低流感病毒RNA聚合酶活性,宿主因子CaMKⅡ和PACT在流感病毒RNA聚合酶的合成过程中起着重要的作用,但PACT的作用要更明显于CaMKⅡ。
Objective To observe the effect of host factors CaMK Ⅱ and PACT on influenza A virus RNA polymerase compared with ribavirin. Methods Dual luciferase reporter gene approach was used to detect relative expression of influenza A virus polymerase in 293T cells, judging the influence of influenza A virus polymerase by treatment with ribavirin and silencing protein CaMK Ⅱ and PACT. Results Silence CaMK Ⅱ and PACT all decreased the relative expression of influenza A virus RNA polymerase. But the inhibition was more obvious by dealing with ribavirin. Conclusion Ribavirin can control the expression of the influenza A virus RNA polymerase. The host factors CaMK Ⅱ and PACT play an important role in the process of synthesis of the influenza A virus RNA polymerase, and protein PACT is more significant than CaMK Ⅱ .
出处
《中国药物警戒》
2016年第1期1-4,共4页
Chinese Journal of Pharmacovigilance
基金
国家自然科学基金(81303290)
北京市自然科学基金(7132141)
中国博士后基金(2015T80208)
中国中医科学院自主选题项目(ZZ2014041
ZXKT15017
自由探索第二批)
