摘要
列当是一类难以防治的恶性根寄生杂草,目前在番茄、向日葵、烟草和瓜类上危害日益严重。列当的种类多且难以区分,明确列当的种类对制定有效防治措施具有重要意义。从新疆维吾尔自治区、河北省、内蒙古自治区和吉林省4个地区采集了19份列当样品,通过形态观察和DNA条形码技术进行种类鉴定并对其亲缘关系进行讨论。结果发现,寄生于新疆番茄上的列当样品为分枝列当,寄生于河北烟草、河北向日葵和吉林向日葵上的列当样品为弯管列当;在系统发育树中分枝列当与弯管列当有明显的系统进化差异性,弯管列当被分为2个大类群,地理区划明显而与寄主植物无关,采集自河北省的7个样品为1个类群,采自吉林省、新疆维吾尔自治区、内蒙古自治区的8个样品为第2个类群,其中第2类群中采自吉林省的4个样品亲缘关系最近。
As noxious root parasitic weeds,species of Orobanche（ broomrape） are hard to control and cause serious losses in many subtropical crops such as tomato,sunflower,tobacco and melon. It is difficult to distinguish the numerous broomrapes yet it is of great importance to correctly identify them to design effective control measures. Nineteen types of broomrapes were collected from Xinjiang Uyghur Autonomous Region,Hebei Province,Inner Mongolia Autonomous Region and Jilin Province. Species were identified by morphologic observation and DNA bar code technology,and their genetic relationships were investigated. Broomrape parasitizing tomato in Xinjiang was Orobanche aegyptiaca Pers.; those parasitizing tobacco and sunflower in Hebei Province and sunflower in Jilin Province corresponded to Orobanche cernua Loefling.There are obvious system evolutionary differences between O. aegyptiaca and O. cernua in the phylogenetic tree. O. cernua was divided into two taxonomical groups with obvious geographical zoning differentiation unrelated to the host plant.Seven types of broomrapes collected from Hebei Province belonged to a group,eight types from Jilin Province,Xinjiang Uyghur Autonomous Region and inner Mongolia autonomous region belonged to the second group. In the second group,four broomrapes collected from Jilin Province have the closest genetic relationship.
出处
《杂草科学》
2015年第3期6-10,共5页
Weed Science
基金
河北省财政项目(编号:F13R10001)
河北省财政基本科研业务费(编号:494-0401-JBN-6440)
关键词
寄生性杂草
列当
形态鉴定
DNA条形码
系统进化
parasitic weed
broomrape
morphological character identification
DNA bar code
phylogeny
