摘要
本试验旨在研究不同剂型丁酸钠对脂多糖(LPS)应激肉鸡血清生化指标、抗氧化和抗炎功能的影响。饲养试验将1 000羽1日龄岭南黄肉鸡随机分为5组,每组4个重复,每重复50羽。对照组饲喂基础饲粮,试验组在基础饲粮中分别添加500 mg/kg粉剂丁酸钠(Na B)及250、500、750 mg/kg包膜丁酸钠(CNa B),试验为期50 d。应激试验分别从对照组、Na B组以及生长性能最佳组(250 mg/kg CNa B组),每重复随机选6只鸡,按2×3因子试验设计,以应激种类(生理盐水和LPS)和饲粮类型(0、500 mg/kg Na B和250 mg/kg CNa B)作为主效因子,各组1/2鸡只于53、56和59 d腹腔注射大肠杆菌LPS(0.5 mg/kg BW),剩余鸡只则注射等量生理盐水,试验为期10 d。结果表明:1)与生理盐水注射组相比,LPS注射组显著降低了血清中葡萄糖和甘油三酯含量(P<0.05),显著提高了血清尿酸含量(P<0.05)。LPS注射下,Na B组和CNa B组中血清葡萄糖含量均显著高于对照组(P<0.05)。2)与生理盐水注射组相比,LPS注射组显著或极显著降低了血清和肝脏中超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性(P<0.05或P<0.01),显著降低了血清过氧化氢酶(CAT)活性(P<0.05);LPS注射下,Na B组和CNa B组血清和肝脏SOD、CAT活性均高于对照组(P>0.05),丙二醛(MDA)含量低于对照组(P>0.05)。LPS注射和饲粮添加丁酸钠对肝脏GSH-Px活性影响存在交互作用(P<0.05),且2种剂型作用效果相类似。3)与生理盐水注射组相比,LPS注射组极显著提高53和59 d血清铜蓝蛋白和白细胞介素-6(IL-6)含量(P<0.01);LPS注射下,Na B组和CNa B组53 d血清铜蓝蛋白含量显著低于对照组(P<0.05)。由此可见,在LPS应激条件下,丁酸钠能改善肉鸡营养代谢,维持机体抗氧化功能,增强抗炎功能,且500 mg/kg Na B和250 mg/kg CNa B(有效含量为75 mg/kg)作用效果基本一致。
The trial was conducted to study on the effects of different type of sodium butyrate on serum biochemical indices,antioxidant and anti-inflammatory function of broilers challenged with lipopolysaccharide（ LPS）. Feeding trial： a total of 1 000 one-day-old Lingnan yellowbroilers were randomly allocated into 5groups with 4 replicates in each group and 50 broilers per replicate. Broilers in control group were fed a basal diet,the others in experimental groups were fed the basal diets supplemented with 500 mg / kg powder sodium butyrate（ Na B）,and 250,500 and 750 mg / kg coated sodium butyrate（ CNa B）,respectively. The LPS injection experiment was conducted followed a 50-day feeding trial,72 fifty-day-old female broilers were selected randomly from control,Na B and 250 mg / kg CNa B group with the best growth performance,with 6 broilers per replicate. And they were allotted into a 2 ×3 factorial arrangement with challenge status（ saline or LPS exposure） and feed diet（ 0,500 mg / kg Na B or 250 mg / kg CNa B） as main factor for endotoxin exposure test.Half of broilers in each group were injected intraperitoneally with E. coli LPS（ 0. 5 mg / kg body weight） at 53,56 and 59 d of age,the remaining broilers were injected intraperitoneally with the same amount of saline as the LPS injected group. The trial lasted for 10 days. The results showed as follows： 1） compared with the saline injected group,the content of glucose and riglyceride in serum was significantly decreased in LPS injected group（ P〈0. 05）,but the serum uric acid content as significantly increased（ P〈0. 05）. The serum glucose content of Na B and CNa B groups injected with LPS was significantly higher than that of control group（ P〈0. 05）. 2） Compared with the saline injected group,the activity of superoxide dismutase（ SOD） and glutathione peroxidase（ GSH-Px） in serum and liver was significantly decreased in LPS injected group（ P〈0. 05 or P〈0. 01）,the serum catalase（ CAT） activity was signif
出处
《动物营养学报》
CAS
CSCD
北大核心
2015年第10期3146-3154,共9页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
国家肉鸡产业技术体系(CARS-42-G19)
浙江省重大科技专项(2013C02010)
关键词
丁酸钠
肉鸡
生化指标
抗氧化
抗炎
sodium butyrate
broilers
biochemical indices
antioxidant
anti-inflammation
