摘要
目的分析Graves病(GD)患者外周血中CD4+CD25+和CD8+CD28-调节性T(Tr)细胞的变化。方法选择该院内分泌科门诊或住院部43例GD患者为研究对象,其中内分泌科门诊确诊的19例GD患者为试验初发组。接受治疗且甲状腺功能恢复正常的24例GD患者为治疗缓解组;选择同期健康体检者20例为健康对照组。采用流式细胞术检测3组外周血中CD4+CD25+和CD8+CD28-Tr细胞水平。结果试验初发组外周血CD4+CD25+Tr细胞水平为(4.56±4.14)%,明显低于健康对照组的(8.84±4.45)%,差异有统计学意义(P<0.05),CD8+CD28-Tr细胞水平为(14.95±5.38)%,与健康对照组的(10.65±6.37)%比较,差异无统计学意义(P>0.05)。治疗缓解组外周血中CD4+CD25+Tr细胞水平为(6.99±6.35)%,与健康对照组比较,差异无统计学意义(P>0.05),但其CD8+CD28-Tr细胞水平为(20.48±6.07)%,高于健康对照组,差异有统计学意义(P<0.05)。结论 CD4+CD25+Tr细胞水平可能与GD发病有关,CD8+CD28-Tr细胞可能与GD病程发展有关。
Objective To analyze the changes of the levels of CD4＋CD25＋and CD8＋CD28-T cells in the peripheral blood in patients with graves disease（GD）.Methods A total of 43 patients with GD were selected as objects in this study,19 cases who were diagnosis in outpatient department were selected into incipience group,24 cases who were treated and the thyroid function return to normal status were selected into alleviation group,meanwhile 20 healthy persons were recruited into control group.The levels of CD4＋CD25＋ and CD8＋CD28-regulator T cells in the peripheral blood were detected by flow cytometry.Results The CD4＋CD25＋regulatory T cell levels in incipience group were（4.56±4.14）% ,which was significant lower than（8.84±4.45）% in the control group（P〈0.05）.The CD8＋CD28- regulatory T cell levels in incipience group and control gruop were（14.95±5.38）% ,（10.65±6.37）respectively,the difference had no significant difference（P〉0.05）,the CD4＋CD25＋ regulatory T cell level in the alleviation group was（6.99±6.35）% ,which had no significant difference with the control group（P〉0.05）,CD8＋CD28- regulatory T cell level in the alleviation group was（20.48±6.07）% ,which was significant higher than that in the control group（P〈0.05）.Conclusion The level of CD4＋CD25＋ regulatory T cell in GD patients indicate that they might play an essential role in the pathogenesis of GD.CD8＋CD28- regulatory T cell plays important roles during the development of the disease in patients with GD.
出处
《检验医学与临床》
CAS
2015年第10期1376-1377,共2页
Laboratory Medicine and Clinic
