摘要
向日葵锈病严重影响向日葵的产量。为了找到抗锈病相关基因，根据已知NBS-LRR型抗病基因保守结构域P-loop和GLPL设计简并引物，以接菌的抗病向日葵品种CM29叶片的cDNA为模板进行扩增。通过克隆转化得到10个具有连续开放阅读框的抗病基因同源序列（RGA），经系统进化分析将其分为TIR-NBS-LRR和non-TIR-NBS-LRR两种类型。对其氨基酸序列进行多重序列比对，结果显示所获得的RGA具有典型的NBS-LRR型抗病基因保守结构域，即P-loop、kinase-2a、kinase-3a和GLPL结构。运用BLASTX分析这些结果，表明其RGA与已知的抗病基因相应保守区域的同源性为18．1％～51．1％，说明它们可能与抗病功能基因具有密切联系。
Sunflower （Helianthus annuus）rust restricts growth and production severely.In order to find resist-ance genes of sunflower to rust disease,degenerated primers were designed based on conserved regions of NBS -LRR genes,P-loop and GLPL,to clone the analogs from infected resistant cultivar CM29 .By phylogenetic analy-sis,10 cloned resistance gene analogs with continuous open reading frame belonged to TIR-NBS-LRR type and non-TIR-NBS -LRR.Multiple sequence alignment of amino acid showed these RGAs had typical domains of NBS-LRR resistance genes,including P-loop,kinase-2a,kinase-3a and GPLP.These RGAs showed great homology with the known resistance genes by BLASTX analysis,indicating the RGCs were highly related to resist-ance genes in sunflower.
出处
《中国油料作物学报》
CAS
CSCD
北大核心
2014年第4期508-512,523,共6页
Chinese Journal of Oil Crop Sciences
基金
国家自然科学基金(31060235
31360422)
旱区作物逆境生物学国家重点实验室开放课题
国家向日葵现代产业技术体系(CARS-16)
关键词
向日葵
锈病
抗病基因同源序列(RGA)
克隆
Helianthus annuus
Puccinia helianthi Schw.
NBS -LRR
Resistance gene analogs
Clone
