摘要
将含基因cry1C的质粒 ,通过电脉冲法转入含基因Cry1C的质粒 ,通过电脉冲法转入含基因Cry1Ab、Cry1Ac和cry2的对小菜蛾具有高毒力的苏云金芽胞杆菌野生菌株YBT 80 3 1中 ,得到转化子BMBY 0 0 3。PCR和SDS PAGE分析显示 ,Cry1C可在其中正常复制、表达 ,但使受体菌部分内源质粒发生丢失。生物测定结果表明 ,转化子BMBY 0 0 3既对甜菜夜蛾有毒力 ,LC50 值为 1 1 78μL/mL ,高于出发菌株YBT 80 3 1 (LC50 1 879μL/mL) ,也对小菜蛾有毒力 ,LC50 值为 1 96 8μL/mL ,低于YBT 80 3 1 ((LC50 1 1 43 μL/mL)。表明cry1C转入后 ,提高了野生菌株YBT 80 3 1对甜菜夜蛾的毒力 ,却降低了对小菜蛾的毒力。
The cry1C gene which is highly toxic to Spodotera exigua was transformed by electroporation into Bacillus thuringiensis wild-type strain YBT-803-1. The transformant BMBY-003 was obtained. The plasmid profile of transformant showed that three plasmids were lost. SDS-PAGE analysis demonstrated that cry1C could express in BMBY-003. But the production of cry1Ac was reduced. The result of bioassay showed BMBY-003 was not only toxic to Spodotera exigua larva (LC 501.178μL/mL)which was higher than that of YBT-803-1(LC 501.879μL/mL),but also toxic to Plutella xylostella(LC 501.968μL/mL),lower than that of YBT-803-1(LC 501.143μL/mL).
出处
《微生物学通报》
CAS
CSCD
北大核心
2002年第3期17-20,共4页
Microbiology China
