摘要
【目的】明确'库尔勒香梨'kfpMYB基因表达高低与萼片脱落和宿存的相关性。【方法】在新疆库尔勒巴格吉格代村梨园于盛花期选取强势树和弱势树各3株,各采30朵花的萼片,共180朵。同时每株树上各标记100朵花,落花7 d后进行调查,记录萼片的宿存与脱落情况。参照艾德莱植物RNA快速提取试剂盒的说明书提取总RNA。以总RNA为模板,按照TakaRa PrimescriptTMRT reagent Kit试剂盒的说明书合成'库尔勒香梨'cDNA第一条链。根据cDNA序列信息设计引物,通过实时荧光定量PCR明确kfpMYB基因在强势树与弱势树中的相对表达量,并用SPSS软件分析田间调查的数据。【结果】kfpMYB基因在强势树2～5序位萼片中的表达量均高于弱势树。kfpMYB基因在强势树和弱势树第2序位的表达量显著高于其他序位的表达量。强势树和弱势树的第2、3序位的宿萼率均显著高于第4、5序位,第5序位的脱萼率显著高于其他序位。不同树势中'库尔勒香梨'萼片kfpMYB基因相对表达量高低与萼片脱落和宿存有相关性,但不显著;在不同序位中,第4序位中'库尔勒香梨'萼片kfpMYB基因的相对表达量与萼片宿存呈显著负相关,第5序位中'库尔勒香梨'萼片kfpMYB基因的相对表达量与萼片脱落呈显著负相关。【结论】'库尔勒香梨'萼片kfpMYB基因的表达量与萼片的脱落和宿存存在相关性,不同树势中的相关性不显著,但在不同序位中存在显著相关。
[Objective]‘Kuerlexiangli'pear is one of the most important agricultural crops in Xinjiang.The calyx of‘Kuerlexiangli'pear may be either persistent or deciduous.A persistent calyx can negatively affect fruit shape,reducing its appeal to customers.Therefore,it is of great importance to reveal the mechanism controlling the calyx persistence.The purpose of this study was to determine the correlation between kfpMYB gene expression and calyx persistence.[Methods]Flowers of‘Kuerlexiangli' pear were collected in an orchard at Bagejigedai village,Korla city,Xinjiang province.The flowers were collected at the full flowering stage.In the flowering period,three‘Kuerlexiangli'pear trees with strong tree potential and weak tree potential were selected,and 30 flowers of 2-5 in the strong tree and the weak tree were collected respectively.Thirty flowers were collected from each tree.After collection,the flowers were immediately frozen in liquid N and then taken to the laboratory where they we stored at -80 ℃ in an ultra low temperature refrigerator.These samples were used to measure gene expression.We also marked 100 flowers on each tree(the second,third,fourth and fifth opened flowers in the clusters).Calyx persistence was determined a week after petal drop.Total RNA was extracted using an Aidelai kit for rapid extraction of plant RNA according to the manufacturer's directions.The concentration of total RNA was detected with a nucleic acid analyzer.The integrity of the total RNA was detected by 1% agarose gel electrophoresis.Total RNA was used as a template to synthesize the first chain of cDNA with a TakaRa Prime Script TMRT Reagent Kit according to the manufacturer's instructions.This step also included the removal of genomic DNA.The primers were designed according to cDNA sequence information of‘Kuerlexiangli'pear kfpMYB gene.The relative expression of the kfpMYB gene in the high vigor trees and the low vigor trees was determined by real-time fluorescence quantitative PCR.The data was analyzed using SPSS softwar
作者
赵欢
王玉凯
牛建新
ZHAO Huan;WANG Yukai;NIU Jianxin(College of Agriculture, Shihezi University · Xinjiang Production and Construction Crops Key Laboratory of Special Fruits and Vegetables Cultivation Physiology and Germplasm Resources Utilization, Shihezi 832003, Xinjiang, China)
出处
《果树学报》
CAS
CSCD
北大核心
2018年第12期1437-1443,共7页
Journal of Fruit Science
基金
国家自然科学基金(31360474)
高等学校博士学科点专项科研基金博导类联合资助课题(2013651810002)
