摘要
目的 探讨氯原酸 (CHA)、抗坏血酸 (AA)、柠檬酸 (CA)和苹果酸 (MA) 4种有机酸对单核 内皮细胞 (MC EC)粘附作用的影响 ,为开发利用天然食物成分有机酸防治动脉粥样硬化提供科学依据。方法 通过原代培养人脐静脉内皮细胞 (EC) ,观察氧化修饰的低密度脂蛋白 (oxLDL) (10 0mg/L) ,不同剂量 (10、2 0、40mg/L)CHA、AA、CA和MA 4种有机酸 +oxLDL(10 0mg/L) ,以及单纯CHA和AA3个剂量 ,分别与EC共培养 12h后MC EC的粘附率。结果 正常对照组MC EC的基础粘附率是(11 35± 1 2 7) % ;oxLDL组粘附率为 (37 2 5± 5 6 3) % ,显著高于正常组 (P <0 0 1) ;有机酸 +oxLDL各组MC EC粘附率与oxLDL组比较 ,有不同程度的降低 ,其中 3个剂量组的CHA +oxLDL和AA +oxLDL的降低率分别为CHA :6 0 81%、72 6 6 %、10 0 96 % ,AA :34 2 9%、6 5 0 6 %、95 76 % ,呈剂量依赖性 ;CA+oxLDL只有高剂量组的MC EC粘附率显著低于oxLDL组 (P <0 0 5 ) ,MA +oxLDL 3个剂量组无明显作用。单纯CHA和AA各个剂量组的MC EC粘附率略低于正常对照组 ,但差异不显著。结论 CHA和AA对oxLDL诱导的MC EC粘附作用具有明显的抑制效应 ,CA作用较弱 ,而MA作用不明显。
Objective This study examined the effects of four organic acids (OA), namely chlorogenic acid (CHA), ascorbic acid (AA), citric acid (CA) and matric acid (MA), on monocyte (MC) adhesion to endothelial cells Methods Original human umbilical vein endothelial cells (EC) were cultured, and the adhesive rates of MC EC were measured after EC were incubated for 12 hours respectively with oxLDL (100 mg/L) in the presence or not of CHA, AA, CA and MA in different concentrations (10, 20, 40 mg/L), as well as with single CHA and AA Results The MC EC adhesive rate of oxLDL group was 37 25%±5 63%, higher than that of normal group (11 35%±1 27%, P <0 01) MC EC adhesive rates of the OA+oxLDL groups were lower at a different extent when compared with oxLDL group The inhibition rates of CHA+oxLDL and AA+oxLDL from low to high dose were: CHA 60 81%, 72 66%, 100 96%; AA 34 29%, 65 06%, 95 76%, respectively, showing a dose effect response During the three doses of CA+oxLDL groups, only high dose group had a lower MC EC adhesive rate than oxLDL group ( P <0 05) However, all the MA+oxLDL group's MC EC adhesive rates were not different from the oxLDL group There was a trend for the MC EC adhesive rates of single CHA or AA in three doses to be lower than that of normal group Conclusion CHA and AA inhibited the monocyte adhesion to endothelial cells induced by oxLDL, the next was CA, but the same effect was not observed in the MA
出处
《中华预防医学杂志》
CAS
CSCD
北大核心
2001年第4期240-243,共4页
Chinese Journal of Preventive Medicine
基金
国家自然科学基金资助项目 (3980 0 12 9)
