摘要
背景:目前因地塞米松注射液广泛及长期使用引起的骨质疏松症发病率逐渐升高,但临床尚没有确切的治疗方法,鹿瓜多肽注射液为常用的促进骨折愈合、提高骨密度的药物。目的:观察鹿瓜多肽注射液在高浓度地塞米松条件下对成骨细胞增殖与分化的影响。方法:将大鼠成骨细胞随机分成3组,地塞米松+鹿瓜多肽注射液组添加1×10-7 mol/L地塞米松注射液、8 mg/L鹿瓜多肽注射液和终浓度为1 g/L的二甲基亚砜;地塞米松组添加与地塞米松+鹿瓜多肽注射液组等量的二甲基亚砜及1×10-7 mol/L的地塞米松注射液;对照组只添加与地塞米松+鹿瓜多肽注射液组等量的二甲基亚砜。96 h后以RT-PCR分析骨钙素的表达,21 d后茜素红染色对比观察钙结节的表达。结果与结论:地塞米松+鹿瓜多肽注射液组与对照组骨钙素的mRNA表达量均高于地塞米松组(P<0.01),地塞米松+鹿瓜多肽注射液组与对照组钙结节表达量也高于地塞米松组(P<0.01),而地塞米松+鹿瓜多肽注射液组与对照组比较差异无显著性意义(P>0.05)。提示鹿瓜多肽注射液可对抗高浓度地塞米松对成骨细胞增殖和分化的抑制作用。
BACKGROUND：The incidence of osteoporosis caused by long-term use of dexamethasone injection is increasing, but there is no exact treatment. Lugua peptide injection is a common drug to promote fracture healing and increase bone density.
OBJECTIVE：To observe the effect of Lugua peptide injection plus high-concentration dexamethasone on osteoblast proliferation and differentiation.
METHODS：The rat osteoblasts were randomly divided into three groups. Lugua peptide plus dexamethasone group was injected with 1×10^-7 mol/L dexamethasone, 8 mg/L Lugua peptide and dimethyl sulfoxide （final concentration 1 g/L）. Dexamethasone group was injected with 1×10^-7 mol/L dexamethasone and dimethyl sulfoxide （final concentration 1 g/L）. Control group was injected with dimethyl sulfoxide （final concentration 1 g/L）. After 96 hours, the expression of osteocalcin was analyzed by RT-PCR analysis;at 21 days, the expression of calcium nodules was observed by alizarin red staining.
RESULTS AND CONCLUSION：The osteocalcin mRNA expression and calcium nodule expression in Lugua peptide plus dexamethasone group and control group were higher than that in dexamethasone group （P〈0.01）, while there was no significant difference between Lugua peptide plus dexamethasone group and control group （P〉0.05）. Lugua peptide injection can prevent the inhibitory effect of high concentrations of dexamethasone on the osteoblast proliferation and differentiation.
出处
《中国组织工程研究》
CAS
CSCD
2014年第15期2314-2319,共6页
Chinese Journal of Tissue Engineering Research
