摘要
绵羊肺腺瘤(OPA)是由β-反转录病毒属绵羊肺腺瘤病毒(JSRV)引起的一种肿瘤性传染病,该病潜伏期长,经呼吸道传播,冬季圈养种羊发病率高,目前无治疗措施,病死率为100%。OPA的持续存在对种羊生产形成了潜在威胁并造成较大经济损失,严重危害养羊业健康发展。所以,对OPA的早期精确诊断是防制本病的前提,尤其是在出入境检验检疫过程中对进出口羊的JSRV检测尤为重要。随着分子生物学技术的不断发展,JSRV分子生物学检测方法也得到不断的创新和改进。作者就近年来针对检测JSRV的聚合酶链式反应(PCR)、核酸探针杂交技术、酶联免疫吸附试验(ELISA)、环介导等温扩增技术(LAMP)等方法的研究概况作一综述,为寻找快速准确并适用于出入境检疫的JSRV检测方法和进一步发展研究新型JSRV检测方法提供参考。
The ovine pulmonary adenomatosis （OPA） is composed of β-reverse transcription virus jaagsiekte sheep retrovirus （JSRV） caused by a tumor infectious disease, the disease has a long incubation, spreads through the respiratory tract, the winter of captive breeding disease incidence rate is high, at present there are no treatment measures, the mortality rate is 100% . The continuing presence of OPA is a potential threat for sheep production and has caused great economic losses, serious harm to the healthy development of sheep industry. Therefore, early precise diagnosis on OPA is the premise of the disease prevention and control, particularly in the entry exit inspection and quarantine process of import and export of sheep JSRV detecting is very important. With the development of molecular biology techniques, JSRV molecular biology detection method has constant innovation and improvement. This review will focus on the detection method of JSRV, polymerase chain reaction （PCR）, nucleic acid hybridization, enzyme linked immunosorbent assay （ELISA）, loop mediated isothermal amplification （LAMP） survey research methods in recent years, to provide reference methods for the detection of JSRV and further development of the research on new JSRV detection methods for rapid, accurate and suitable in the entry and exit inspection and quarantine process.
出处
《中国畜牧兽医》
CAS
北大核心
2014年第4期236-241,共6页
China Animal Husbandry & Veterinary Medicine
基金
国家自然科学基金项目(31101788
31260593)
关键词
绵羊肺腺瘤
绵羊肺腺瘤反转录病毒
检测方法
ovine pulmonary adenomatosis
jaagsiekte sheep retrovirus
detection method
