摘要
本研究采用聚丙烯酰胺凝胶垂直板型电泳法 ,分析了 3种瘤背豆象属豆象 :绿豆象 Callosobruchuschinensis L.、灰豆象 C.phaseoli ( Gyllenhal)、鹰嘴豆象 C.analis ( Fabricius)幼虫的酯酶同工酶。从酶谱的酶带数、迁移率、酶活性强弱 (酶带染色深浅 )、酶含量 (酶带宽窄 )等方面的比较发现 ,3种瘤背豆象属豆象幼虫的酯酶同工酶酶谱具有明显的差异。
The method of vertical plate type of polyacrylamide gel electophoresis had been used to analyse the lipase isoenzymes of bean weevil larvae of three species: Callosobruchus chinensis L. ,C. phaseoli (Gyllenhal) ,and C. analis(Fabricius). The distinct difference in zymograms of all three species had been found by comparing band number, migration rate, strength of enzymic activity(depth of band dyeing),the enzyme content(width of enzymic band ),etc. in lipase isoenzyme of bean weevil larvae. The possibility of identifying the species of bean weevils by using the electophoresis method on larval lipase isoenzyme was discussed.
出处
《昆虫知识》
CSCD
北大核心
2001年第1期62-64,共3页
Entomological Knowledge
