摘要
以中烟100为材料,研究了茉莉酸甲酯(MeJA)喷施后,烟草叶面腺毛密度类型、腺毛分泌物合成积累及叶片对蚜虫抗性的变化规律,探讨了烟草叶面腺毛的防御反应模式和作用机制。结果表明,MeJA处理后,烟草叶片对蚜虫的驱避作用明显增强,新生叶片叶面腺毛密度增加,尤其是长柄多细胞腺头腺毛密度的变化达到显著水平。GC/MS分析表明,MeJA处理使叶面分泌物含量增加,并在处理后第7天达到最大值。其中,西柏烷类二萜化合物含量增幅最大。但RT-PCR分析发现,西柏三烯一醇合成酶基因(CBT)、西柏三烯一醇羟化酶基因(CYP71D16)在转录水平上并没有明显变化,表明烟草叶面腺毛物质的合成和分泌可能具有复杂的转录后调控机制。
In order to explore the defense mode and mechanism of tobacco leaf trichome, Methyl jasmonate （MeJA） was choose to spray on tobacco seedling. After the treatment, trichome morphology, leaf surface chemical composition, gene expression and leaf resistance response to aphid were studied. Scanning electron microscope observation found that the trichome density of the new born leaves increased after MeJA treatment, especially the secreting type which has multicellular head. GC/MS analysis results showed that the content of leaf surface chemical substances increased, which reached the maximum value at the 7th day after treatment. Although the content of cembranoids, which was synthesized and secreted only by trichomes, changed greatly, the expression of its synthesis related genes, CBT and CYP71D16, did not show much difference during the MeJA treatment. It suggests that the complex posttranscriptional regulation mechanism in tobacco glandular hairs existed, which should be explored further.
出处
《中国烟草科学》
CSCD
2013年第5期83-88,共6页
Chinese Tobacco Science
基金
中国烟草总公司科技重点项目(110200902045)
关键词
烟草
腺毛
MEJA
分泌
防御反应
tobacco
trichome
MeJA
secretion
defense response
