摘要
利用30对SSR引物,检测2005—2009年参加国家区域试验的1068份大豆品种（系）的位点纯合度。每年区试品种（系）的平均纯合度为94.9%~97.6%,纯合度高于85%和90%的品种（系）所占比例分别为95.0%和91.4%,纯合度低于85%的品种（系）有42份（占3.93%）,主要为北方春大豆和黄淮夏大豆。位点纯合度低于85%的参试品种（系）的产量分析结果表明,只有11份品种（系）比对照增产5%以上,20份比对照减产0.04%~13.08%;与纯合度为100%的品种（系）比较发现,位点纯合度较低的品种（系）在区试中产量也较低。建议国家区试品种（系）纯合度标准不低于90%,以保证审定品种的特征特性,为大豆新品种的持续推广利用提供科技支撑。
Genomic homozygosity of seeds is very important for protection of breeder’s right and sustainable production of agriculture. Highly informative microsatellite （SSR） markers, widely used in soybean genetic mapping and genetic diversity research, are useful for soybean genomic analysis. Thirty pairs of SSR primers were used to test the DNA of 1 068 soybean varieties （lines） from regional trials of China. The average SSR locus homozygosity for each year ranged from 94.9% to 97.6% each year. Among the 1 068 varieties （lines） in China regional test identified in five years, the soybean varieties （lines） with homozygosity higher than 85% and 90% accounted for 95.0% and 91.4%, respectively. Only 42 （3.93%） of the varieties （lines） had homozygosity less than 85%, which were mostly from North spring and Huanghuai summer soybean planting areas. Among the 42 varieties （lines） with homozygosity less than 85% only 11 had the yield 5% more than the controls, 20 had a yield reduction of 0.04% to 13.08%. Compared with those with 100% homozygosity, varieties with lower SSR loci homozygosity also had lower yield in regional trials. It is suggested that criterion of soybean homozygosity in the national regional test should not be less than 90% so as to insure the protection and sustainable utilization of new soybean varieties （lines） in production.
出处
《作物学报》
CAS
CSCD
北大核心
2012年第10期1760-1765,共6页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30971801)
国家重点基础研究发展计划(973计划)项目(2009CB118400)资助
关键词
区域试验
大豆
SSR位点
纯合度
Regional test
Soybean [Glycine max （L.） Merr.]
SSR loci
Homozygosity
