摘要
以亚硫酸盐蔗渣浆为研究对象,研究了底物浓度、外源添加物种类和浓度对纤维素酶解工艺的影响以及PEG6000强化酶解效率的作用机理。研究结果表明,纤维素酶用量15 FPIU/g(以绝干纤维素计,下同)、β-葡萄糖苷酶用量30 CBU/g,纤维素质量浓度80 g/L条件下水解48 h,葡萄糖质量浓度达72.51 g/L,葡萄糖得率、纤维素酶解得率和总糖得率达81.58%、86.79%和84.23%。PEG6000可有效强化酶解,添加量为2 g/L时,水解48 h葡萄糖质量浓度可升至78.54 g/L,葡萄糖得率、纤维素酶解得率和总糖得率达88.36%、95.02%和92.54%。添加2 g/L的PEG6000使纤维素酶Celluclast1.5 L滤纸酶活力提高到原酶活力的117.33%;同时50℃,pH值4.8,保温48 h,残余酶活力同比增加38.99%。
In order to optimize the hydrolysis conditions of bagasse sulfite pulp, the influences of substrate concentration, species and concentration of exogenous additives on hydrolysis were investigated in present study. The results showed that the sample with 72.51 g/L glucose concentration, 81.58% glucose yield, 86.79% hydrolysis yield and 84.23% total sugar yield were obtained by using 15 FPIU/g cellulase and 30 CBU/g/3-glucosidase with 80 g/L cellulose after 48h hydrolysis. Addition of PEG6000 could enhance the hydrolysis process remarkably. The glucose concentration reached 78.54 g/L after 48 h of hydrolysis in pres- ence of 2 g/L PEG6000. Meanwhile, glucose yield, hydrolysis yield and total sugar yield increased to 88.36% , 95.02% and 92.54% , respectively. In addition, 117.33% increase in enzyme activity and 38.99% in stability of Celluclast 1.5L were observed when 2g/L PEC,6000 was applied.
出处
《林产化学与工业》
EI
CAS
CSCD
北大核心
2012年第3期28-32,共5页
Chemistry and Industry of Forest Products
基金
国家自然科学基金资助项目(31070513)
江苏省高校自然科学研究重大项目(10KJA22019)
2011年江苏省普通高校研究生科研创新计划(CXLX11_0527)
