摘要
目的:研究白藜芦醇(Res)对呼吸道合胞病毒(RSV)感染BALB/c小鼠肺泡灌洗液(BALF)不同时间点肿瘤坏死因子-α(TNF-α),白细胞介素-1β(IL-1β),白细胞介素-6(IL-6)表达的调控趋势。方法:RSV滴鼻感染BALB/c小鼠,白藜芦醇灌胃给药进行干预,并于首次滴鼻后24,72,144 h取各组小鼠支气管肺泡灌洗液,ELISA法检测其中TNF-α,IL-1β,IL-6含量变化及Res的干预效果。结果:RSV感染BALB/c小鼠24 h后,BALF中TNF-α,IL-1β,IL-6表达较正常组均明显升高(P<0.01),而Res组TNF-α(P<0.01),IL-1β(P<0.05),IL-6(P<0.01)表达较RSV组明显下调;RSV感染BALB/c小鼠72 h后,BALF中TNF-α(P<0.05),IL-1β(P<0.01),IL-6(P<0.01)表达较正常组高,但TNF-α,IL-1β,IL-6总体表达量较24 h组下降趋势明显,Res组IL-1β,IL-6表达较RSV感染组降低(P<0.05),而TNF-α表达与RSV感染组比较无显著性差异;RSV感染BALB/c小鼠144 h后,BALF中IL-1β(P<0.01),IL-6(P<0.05)表达较正常组高,而TNF-α表达较正常组无差异,Res组TNF-α,IL-1β,IL-6表达与RSV感染组比较无显著性差异。结论:白藜芦醇能显著抑制RSV感染BALB/c小鼠早期肺泡灌洗液内炎性细胞因子TNF-α,IL-1β,IL-6的过度表达,且随着感染时间的延长,能使TNF-α,IL-1β,IL-6的表达维持在相对较低的水平。
Objective： To study the regulation trend of resveratrol on TNF-α, IL-1β, IL-6 expressions in bronchoalveolar lavage fluid （BALF） of RSV-infected BALB/c mice at different time points. Method： RSV-induced BALB/c mice were orally administered with resveratrol. Their BALFs were collected at 24, 72 and 144 h after the first nasal drip with RSV to detect the level of TNF-α, IL-1β, IL-6 by EILSA. Result： The expression of TNF-α, IL-1β and IL-6 in BALF increased significantly compared with the normal group （ P 〈 0. 01 ） after 24 hours of RSV infection, while the expression of TNF-α （ P 〈 0. 01 ）, IL-1β （ P 〈 0. 05 ）, IL-6 （ P 〈 0. 01 ） in the resveratrol group decreased notably compared with the model group. After 72 hours of infection with RSV, although the expression of TNF-α （P 〈0. 05）, IL-1β （P 〈0. 01 ） and IL-6 （P 〈0. 01 ） in BALF in model group were higher than those in the normal group, they were much more lower than at 24 h. The expression of IL-1β and IL-6 （ P 〈 0. 05 ） in the resveratrol groups were down-regulated significantly, but no difference had been shown in TNF-α expression compared with the RSV infection group. After infection with RSV for 144 h, the expression of IL-1β （P 〈0. 01 ） and IL-6 （P 〈0. 05） in BALF in the model group were higher than those in the normal group, but there was no difference in the secretion of TNF-α. The expression of TNF-α, IL-1β and IL-6 showed also no remarkable difference between the resveratrol groups and the RSV infection group. Conclusion： Resveratrol can inhibit the over expression of inflammatory factors TNF-α,IL-1β,IL-6 in bronchoalveolar lavage fluid of RSV-induced BALB/c mice and keep them at a low level with the passing of infection time.
出处
《中国中药杂志》
CAS
CSCD
北大核心
2012年第10期1451-1454,共4页
China Journal of Chinese Materia Medica
基金
国家自然科学基金项目(81072840)
全国高等学校博士学科点专项科研基金项目(20093237110006)
