摘要
以终浓度为10μmol/L CdCl2对体外培养淋巴细胞进行染毒,分别培养12、24、36、48和72h时收集细胞,提取体外培养脾淋巴细胞膜,采用试剂盒比色的方法,分别检测了细胞膜GSH-Px、SOD的活性、膜MDA和SA含量及培养液LDH的活性,来观察镉性细胞损害时细胞膜的抗氧化功能和通透性。结果显示,随着镉作用时间的延长,淋巴细胞膜GSH-Px、SOD的活性降低,MDA生成量增加,试验组与对照组差异显著(P<0.05)或极显著(P<0.01);培养液中LDH活性升高,膜SA含量降低。表明镉可使淋巴细胞膜的抗氧化功能降低,膜完整性被破坏。
To research the influence of cadmium on chicken spleen lymphocyte membrane antioxidant function,spleen lymphocyte was contaminated with 10 μmol/L CdCl2.After cultured 12,24,36,48 and 72 h,contaminated cells were collected separately.By comparing the colors of kits,GSH-Px of membrane,activity of SOD,content of MDA in membrane were determined to observe membrane antioxidant function when spleen lymplocyte the was damaged by cadmium.The results showed that the cadmium exposure induced,the decreased lymphocyte membrane GSH-Px,SOD activity,and the increased MAD.There are obvious distinctions between tested groups and control groups（P0.05 or P〈0.01）.Cadmium can inhibited spleen lymphocyte membrane antioxidant function,destroyed membrane oxidant integrity.
出处
《中国兽医学报》
CAS
CSCD
北大核心
2012年第5期744-747,共4页
Chinese Journal of Veterinary Science
关键词
镉
鸡
脾淋巴细胞膜
抗氧化功能
cadmium
chicken
spleen lymphocyte membrane
antioxidant
