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一种快速测定毛竹半纤维素中糖醛酸含量的方法 被引量:2

A Quick Method for Quantitative Determination of Uronic Acid in Hemicellulose of Moso Bamboo
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摘要 采用间羟基联苯比色法测定毛竹半纤维素中葡萄糖醛酸含量,并研究了四硼酸钠/硫酸、间羟基联苯溶液添加量及沸水浴时间对测定结果的影响,建立了适合于毛竹半纤维素中糖醛酸含量的测定条件,同时证明了该方法测定毛竹半纤维素中葡萄糖醛酸的稳定性、可重复性和精密度。 Arabinoglucuronoxylan is the main component in the hemicellulose of moso bamboo.The content of glucuronic acid which is as the main side chain can reflect the structure of the hemicellulose.In this paper,a method for the determination of glucuronic acid in hemicellulose of moso bamboo with m-hydroxydiphenyl was used.The amounts of tetraborate/H2SO4,m-hydroxydiphenyl and the boiling time were studied.This method was proved simple,accurate and reproducible.
出处 《中国造纸》 CAS 北大核心 2012年第3期16-19,共4页 China Pulp & Paper
基金 国家自然科学基金项目(30960304)资助
关键词 毛竹半纤维素 葡萄糖醛酸 间羟基联苯比色法 hemicellulose moso bamboo glucuronic acid method of m-hydroxydiphenyl
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  • 1BITTER T. A modified uronic acid carbazole reaction [J].Anal Biochem, 1962,4: 330. 被引量:1
  • 2BLUMENKRANTZ N, ASBOE-HANSEN G. New method for quantitative determination of uronic acids [J]. Anal Biochem, 1973,54:484 - 489. 被引量:1
  • 3MESEGUER I. Extraction and colorimetric quantification of uronic acids of the pectic fraction in fruit and vegetables [J]. J Food Composition and Analysis, 1998,11:285 -291. 被引量:1
  • 4BIANCA M. A microtiter plate assay for the determination of uronic acids[J]. Anal Biochem, 1998,257: 107 - 111. 被引量:1
  • 5陈鸿英,朱永智,吴乃居,孙燕.朝鲜淫羊藿多糖的含量测定[J].中草药,2003,34(9):810-811. 被引量:18

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