摘要
本试验在茎尖培养基中分别加入3种浓度的板蓝根（1．0g/l、2．0g/l、4.0g/l）和3种浓度的三氮唑核苷酸（10mg／l、20mg／l、40mg／l），研究了不同药剂浓度对葡萄试管苗茎尖的影响。试验结果表明两种药剂对葡萄茎尖生长和分化率均有抑制作用，而且随着浓度的增加，抑制作用也增强。经板蓝根2．0g／l和4.0g／l处理获得的茎尖试管苗，移栽田间后，经双链RNA技术分析检测，未检测出双链RNA.
In the study of grape virus-free, different concentration medicine BanLanGen (l.0g/L, 2.0g/L, 4.0g/L)andnucleotide trinitro (l0mg/L. 20mg/L. 40mg/L)were applied into medium of tip culture, the effect of the differentmedicine on the growth of vitro grape tip and leafroll virus were studied. The result shows that both medicine haveinhibiting effect on grape tip growth and its differentiation, and the inhibiting effect strenghthened as the concentra-tion increased. The result of Virus detection with Double-RNA method to the young p1ant of vitro tip culture treatedwith BanLanGen 2.0g/L and 4.0g/L and transplanted into field shows that there is no Double-RNA existing.
出处
《中外葡萄与葡萄酒》
2000年第1期15-17,共3页
Sino-Overseas Grapevine & Wine
