摘要
通过微波蒸馏-固相微萃取-气相色谱-质谱方法测定罗非鱼肉中土腥味物质——土臭素和2-甲基异莰醇的含量。对微波蒸馏功率、微波蒸馏时间、载气气流等实验参数进行优化。结果表明:确立的最佳条件为微波功率360W(仪器额定功率的40%)、微波时间6min、载气流速60mL/min;在优化条件下,罗非鱼肉中的土腥味物质能最大限度地被检测出来,土臭素和2-甲基异莰醇的检测限分别达到了0.044μg/kg和0.095μg/kg,且它们在0.5～20μg/kg范围内呈较好的线性关系,r2分别达到了0.998和0.991,实验中土臭素和2-甲基异莰醇的回收率分别为42.7%、61.9%;最终利用该方法测得所购罗非鱼肉中土臭素和2-甲基异莰醇的含量分别为4.97、1.21μg/kg。
An analytical method was established for determining geosmin（GEO） and 2-methylisoborneol（MIB） as off-odor compounds in freshwater fish using gas chromatography-mass spectrum（GC-MS） based on microwave assisted distillation followed by solid phase microextraction.Such sample preparation parameters as microwave power,distillation time and carrier gas flow rate were optimized to be 360 W（40% of the maximum power）,6 min,and 60 mL/min,respectively.Under the optimized conditions,the detection limits were 0.044 μg/kg for GEO and 0.095 μg/kg for MIB.A good linearity was observed for GEO and MIB over the concentration range of 0.5 to 20 μg/kg with r2 of 0.998 and 0.991,respectively.The recoveries were 42.7% for GEO and 61.9% for MIB.Using this method,the contents of GEO and MIB in a commercially available tilapia meat sample were determined to be 4.97 μg/kg and 1.21 μg/kg,respectively.
出处
《食品科学》
EI
CAS
CSCD
北大核心
2011年第22期188-191,共4页
Food Science
基金
国家农业产业技术体系项目(CARS-49)
国家农业科技成果转化项目(2010GB23260577
2009GB2E200303
2010GB2E000335)
广东省科技计划项目(2009A020700004
2008A020100006
2009B020201003)
广东省海洋渔业科技推广项目(A200899B02
A200901C01)
农业部中央级公益性科研院所基本科研项目(2010YD07)
关键词
土腥味物质
微波蒸馏
罗非鱼
土臭素
2-甲基异莰醇
off-flavor compounds
microwave-assisted distillation
tilapia
geosmin
2-methylisoborneol
