摘要
对纤维杆菌x3-14固态降解花生秸秆的发酵条件进行研究。以花生秸秆粉为底物,利用摇瓶液体发酵的方式考察易利用的碳源(蔗糖)和氮源(尿素与硫酸铵)对纤维杆菌x3-14产纤维素酶的影响。结果表明,添加适量的蔗糖和硫酸铵能显著地促进各纤维素酶系(葡萄糖内切酶、葡聚糖外切酶和β-葡萄糖苷酶)的生成。为提高纤维杆菌x3-14固态降解花生秸秆的效果,选取蔗糖添加量、硫酸铵添加量以及含水量和接种量4个影响因子进行优化,结果表明,在干重为10 g的花生秸秆中添加2 g的蔗糖和0.5 g的硫酸铵、接入1 mL的菌悬液、物料的含水量控制在60%时,花生秸秆降解率可达41.27%。
The solid-state fermentation conditions for peanut straw biodegradation by Cellulomonas x3-14 was investigated in this paper. Firstly, with peanut straw powder as the substrate, the effects of easy-using carbon source （sucrose） and nitrogen source on cellulase production were performed, and the results revealed that the addition of appropriate sucrose and （NH4）2SO4 had a significantly positive effect on the biosynthesis of endoglucanase, exoglucanase and β-glucosidase.The solid-state fermentation parameters was then optimized to improve the degradation rate of peanut straw. As a result, the optimal solid-state fermentation conditions in 100-mL of shake flask were as follows： 10 g of dried peanut straw, 2 g of sucrose, 0.5 g of （NH4）2SO4, 1 mL spore suspension of inoculation quantity and 60% of moisture content. In the case of the optimal conditions, the degradation rate of peanut straw reached 41.27%.
出处
《广东农业科学》
CAS
CSCD
北大核心
2011年第22期9-11,共3页
Guangdong Agricultural Sciences
基金
"十一五"国家科技支撑计划项目(2009BADC6B04)
