摘要
目的建立顶空气相色谱内标法测定血液中乙醇含量的快速分析方法。方法取加入正丙醇、叔丁醇内标液的待检测的全血样品0.50mL 2份和加入乙醇、正丙醇、叔丁醇混合内标液的空白全血0.50mL 2份,直接顶空进样。在选定的色谱条件下,血中的乙醇能与样品中可能存在的主要干扰成分实现基线分离,空白全血中的乙醇、正丙醇、叔丁醇分别作为对照[5]。结果顶空气相色谱内标法采用醇类的易挥发性,以叔丁醇作为内标,用顶空气相色谱、火焰离子化检测器进行检测;经与平行操作的醇类标准品比较,以保留时间定性、峰面积定量来对醇类进行分析,用内标法以醇类对内标物的峰面积比进行定量。在0.1～400.0mg/100mL范围内线性良好,检出限为0.1mg/100mL。结论顶空气相色谱内标法直接顶空进样,不受进样误差干扰,结果稳定,操作方便,分析时间短、线性关系良好、灵敏度高,适用于全血中乙醇含量的测定。
Objective To propose a rapid analysis method for the determination of ethanol content in blood by headspace gas chromatography（HSGC）.Methods We took 0.50 mL of two blood samples together with 1-propanol,tert-butyl alcohol internal standard and 0.50 mL of two blank blood samples mixed with ethanol,1-propanol,tert-butyl alcohol internal standard,then did the automatic headspace injection.In the selected HSGC conditions,ethanol of blood with the main interference components might be presented in the samples and achieved baseline separation.Ethanol,1-propanol,tert-butyl alcohol of blank blood samples were contrasted respectively.Results The HSGC（internal standard method） took the high volatility of alcohols and tert-butyl alcohol as internal standard,and used HSGC,detector of flame ionization to do the test.Compared with the alcohols standard samples operating parallelly,the retention time was used as ration and the quantitative of peak area was used to analyze alcohols.The internal standard method was used for peak area of internalstandard method.Good linearity of alcohols was detected within the of 0.1-400.0 mg/100 mL.Conclusion The method is rapid,sensitive and suitable for detecting trace amounts of alcohol in blood.The result is not affected by the error of injection.
出处
《检验医学与临床》
CAS
2011年第22期2761-2762,共2页
Laboratory Medicine and Clinic
关键词
乙醇
自动顶空进样
气相色谱
内标法
ethanol
auto-headspace injection
gas chromatography
internal standard method
