摘要
为寻找适于中国人Ｗｉｌｓｏｎ氏病（ＷＤ）基因连锁分析的遗传标记及制定ＷＤ基因所在区域的遗传图谱，我们应用Ｄ１３ｑ１４－２１区域４个ＤＮＡ标记对７５名无亲缘关系个体及９个ＷＤ家系成品进行连锁分析。结果发现４个ＤＮＡ标记中３个标记等位片段与白种人相同而杂合率相异，其中２个ＤＮＡ标记Ｄ１３Ｓ３１，Ｐ１２３Ｍ１．８与ＷＤ基因存在紧密连锁关系。其遗传顺序为：着丝点－Ｒｂ－Ｄ１３Ｓ３１－ＷＮＤ。
n this paper , to obtain DNA markers more closely linked to Wilson's disease gene (WD) in Chinese and constructed a genetic map of DNA markers within D13q14-21 which will refine the location of WD , the authors analyzed the segregation of 4 DNA markers within D13q14-21 in 75 unrelated normal Chinese individuals and members of 9 Chinese families with WD. The DNA analysis revealed the polymorphic alleles of 3 markers were the same in Chinese as in Caucasians but the allele frequencies were quite different , we confirmed a tight linkage between WD gene and 2 markers D13S31 , Rb. The genetic order was as follows : centromere - Rb - D13S31 -WND.
出处
《中山大学学报（医学科学版）》
CAS
CSCD
1995年第S1期15-17,27,共4页
Journal of Sun Yat-Sen University:Medical Sciences
基金
国家教委博士点基金
关键词
肝豆状核变性/遗传学
限制性片区长度多态性
染色体图
epatolenticular degeneration/genetics
restriction fragment length polytnorphism
chromosome mapping
