摘要
目的建立曼氏迭宫绦虫动物模型,观察其生活史。方法从感染蛇体内分离曼氏裂头蚴,感染终宿主(猫)和中间宿主(剑水蚤、蝌蚪),以观察其从虫卵孵出幼虫、感染第一、二中间宿主剑水蚤、蝌蚪和终宿主猫的生活史过程。结果感染猫12d后粪检发现虫卵,经解剖猫获得成虫、虫卵;虫卵在室温培养12d孵出钩球蚴,感染剑水蚤6d后发育为原尾蚴;感染蝌蚪后第28d发现裂头蚴。结论成功建立曼氏迭宫绦虫动物模型,裂头蚴在猫体内成熟时间仅12d。
To set up an animal model,and observe the life cycle,Sparganum mansoni were isolated from snake.Animal model of definitive host were set up by peroral infection of S.mansoni to cats.Animal model of intermediate host were set up by infection of coracidia to Cyclops,and procercoid to tadpoles.The whole life cycle process including egg incubation,infection of cats,cyclops,and tadpoles were observed.It was found that eggs of S.mansoni were found from fecal specimens of cats 12 days after the infection.Adult and eggs of S.mansoni were acquired by dissecting cat.Coracidia were found 12 d after incubating eggs at room temperature.Coracidia developed to procercoid 6 d after cyclops infection.S.mansoni was found in tadpoles 28 d after infection.In conclusion,animal model of definitive host and intermediate host of S.mansoni are successfully established.The whole life cycle process needs at lest 2 months,and the time of maturation in cat is 12 days.
出处
《中国人兽共患病学报》
CAS
CSCD
北大核心
2011年第2期152-153,共2页
Chinese Journal of Zoonoses
基金
河南省医学科技攻关计划项目(No.200903151)资助
