摘要
目的:观察六味地黄汤对糖尿病(DM)大鼠模型肾功能及微小RNA192(microRNA192,mi R-192)、Ⅰ型胶原(collagenⅠ)和转化生长因子-β1(TGF-β1)表达的影响。方法:链脲佐菌素(STZ)腹腔注射法诱导建立大鼠DM模型,模型成功后随机分为3组:模型对照组、卡托普利组、六味地黄汤组,另设正常对照组。治疗8周,观察治疗期间及治疗后大鼠一般状况、血糖、24h尿蛋白(24hUpro)、肌酐、尿素氮、肾质量、相对肾质量(KW/BW),并用实时荧光定量PCR测定肾脏mi R-192、collagenⅠ和TGF-β1mRNA的表达情况。结果:造模3组均出现DM及肾脏损害,模型组肾脏mi R-192、collagenⅠ、TGF-β1mRNA的表达明显增加。各治疗组DM大鼠肾功能改善、24h Upro明显减少(P<0.05,P<0.01),肾质量及KW/BW明显降低(P<0.05,P<0.01),mi R-192、CollagenⅠ、TGF-β1mRNA表达下调;六味地黄汤降血糖的疗效优于卡托普利(P<0.01)。结论:六味地黄汤可以减少mi R-192、collagenⅠ、TGF-β1的产生,延缓糖尿病肾病进程。
OBJECTIVE To investigate the effects of Liuwei Dihuang Tang on renal function and expression of miR-192 and collagen Ⅰ in diabetic rats. METHODS The diabetic rat models were induced by intraperitoneal injection of streptozotocin（STZ） and randomly divided into model control group, captopril （Capoten） group and Liuwei Dihuang Tang group. Some normal oth er rats were used as normal control group. All rats were treated with corresponding drugs for 8 weeks. During and after the treatment, the general state , blood glucose levels , total urinary protein of the 24 h, serum creatinine and blood urea nitrogen contents, kidney weight and relative kidney weight were measured. The mRNA of miR-192, TGF-β1 and collagen I in the kidney also were detected by quantitative real-time PCR. RESULTS Diabetes mellitus and renal lesions occurred in the three model groups. The expression of miR-192, TGF-βI and collagen I mRNA of the kidney in diabetic rats increased obviously. Liuwei Dihuang Tang could improve the general state and renal function of the diabetic rats, decrease the blood glucose levels and the excretion rate of the 24 hour urine protein , reduce the expression of miR-192 ,TGF-β1 and collagen Ⅰ mRNA in kidney. CONCLUSION Liuwei Dihuang Tang suppressed synthesis of miR-192 and collagen Ⅰ. Rehmanniae Decoction of Six Ingredients could ameliorate renal lesions in diabetic rats.
出处
《中国医院药学杂志》
CAS
CSCD
北大核心
2010年第18期1531-1534,共4页
Chinese Journal of Hospital Pharmacy
