摘要
目的:探讨艾迪注射液诱导食管癌凋亡的途径。方法:收集食管鳞状细胞癌病人46例,艾迪组及对照组各23例,艾迪组术前给予艾迪注射液80mL/d,静脉滴入,连用7天,对照组不用,两组术前其它处理相同。术后完成常规病理检查后,应用组织芯片技术,采用免疫组化法检测凋亡调控基因Bcl-2、Bax及NF-κBP50的表达。结果:Bcl-2、Bax、NF-κBP50在艾迪组的阳性率分别为39.1%、56.5%、47.8%;在对照组的阳性率分别为78.3%、17.4%、87.0%。两组相比较,Bcl-2、NF-κBP50在艾迪组的表达阳性率比对照组降低(P<0.05),Bax在艾迪组的表达阳性率比对照组增高(P<0.05)。NF-κBP50与Bcl-2表达有正相关性(rs=0.358,P<0.05)。结论:艾迪注射液可以通过抑制NF-κB的活化,下调Bcl-2蛋白的阳性表达,诱导癌细胞凋亡,这可能是其诱导食管癌细胞凋亡的途径之一。
Objective： To explore the pathway of Aidi injection inducing apoptosis of esophageal squamous cell carcinoma（ESCC）.Methods： 46 ESCC patients were divided into Aidi group and control group,each of which included 23 subjects.Patients of Aidi group were given 80ml/d,intravenous injection of Aidi for 7 days before surgery,while patients of control group were not given any Aidi injection.Other related therapeutic medicines were as same in both groups.After completing ordinary HE staining detection,tissue microarray （TMA） was prepared and performed using immunohistochemistry （IHC） method.The apoptosis regulating genes such as Bcl-2 and Bax as well as NF-κB were detected by using immunohistochemistry staining.Results： Regarding to indexes related to apoptosis,the positive expression for Bcl-2,Bax and NF-κBP50 in the experiment group were 39.1%,56.5%,and 47.8% respectively,while in the control group were 78.3%,17.4% and 87.0% respectively.The positive rates of Bcl-2 and NF-κBP50 in the experiment group were significantly lower than those in the control group（P〈0.05）.The rate of Bax in the experiment group was significantly higher than that in the control group（P〈0.05）.There was a positive correlation between NF-κBP50 and Bcl-2（rs=0.358,P〈0.05）.Conclusions： Aidi injection could induce apoptosis of ESCC by inhibiting the activity of NF-κB then downregulating the expression of Bcl-2.It was possible pathway of Aidi injection inducing apoptosis of ESCC.
出处
《辽宁中医药大学学报》
CAS
2010年第5期54-56,共3页
Journal of Liaoning University of Traditional Chinese Medicine
基金
江苏省社会发展基金资助项目(BS2002060)
关键词
艾迪注射液
食管癌
凋亡
Aidi injection
esophageal squamous cell carcinoma
apoptosis
