摘要
研究了菜用大黄组培苗开瓶炼苗过程中叶片气孔的特性。结果表明，菜用大黄组培苗经直接开瓶炼苗0～14d，气孔大小、气孔长径、气孔宽径随开瓶炼苗时间的延长而逐渐减小，并趋向稳定；气孔的关闭率随开瓶时间的延长而迅速增加，达到76．5％；14d炼苗结束后，叶表皮细胞密度显著增加，气孔密度与气孔指数稍有增加，但不显著。闭瓶炼苗7d、然后开瓶炼苗7d可以较快恢复菜用大黄组培苗叶片气孔关闭的适应能力；显微观察发现，不同开瓶时间处理对叶片表皮气孔的细胞结构有明显影响，有光环境与无光环境对炼苗最终没有显著影响。
Tissue-cultured plantlets were exposured to the air to study the effects of opening vellel lid on the stomatal traits of rhubarb during acclimatization. During the 0--14 days acclimatization, the stomatal size, it＇s length and width decrease, then tend to stabilize with the time prolonged. Stomatal closure rate substantially increases, reaching 76. 5%. After 14 days＇ experience,leaf epidermis cells density increased significantly, stomatal density and stomatal index slightly increase. Vessel lid closing 7 d and vessel lid opening 7 d could recover the stomatal function rapidly as soon as possible. Microscopic observation demonstrated that there are great differences in stomatal structure among leaves from the plantlets with different opening-time treatments,while there are no significant difference for exercising plantlets under light condition or under dark.
出处
《河南农业科学》
CSCD
北大核心
2010年第5期83-87,共5页
Journal of Henan Agricultural Sciences
基金
河南省杰出青年基金(074100510019)
河南科技学院科研启动基金(050103)
教育部归国留学人员科研启动基金(2005)
河南省归国留学人员科研启动基金(2005)
关键词
菜用大黄
组培苗
炼苗
气孔
Culinary rhubarb
Tissue cultured plant
Acclimatization
Stomata
