摘要
目的探讨环境雌激素壬基酚(NP)对雌性大鼠肝脏、肾脏及子宫的氧化损伤作用。方法清洁级SD雌性大鼠24只,随机分为3组,NP低剂量组(50mg/kg),NP高剂量组(200mg/kg)及对照组,每组8只。每天灌胃染毒不同浓度的壬基酚,对照组大鼠每天给予玉米油(1ml/100g)灌胃,30d后处死大鼠,取肝脏、肾脏及子宫,制成10%匀浆,对肝、肾超氧化物歧化酶(SOD)活力及丙二醛(MDA)含量和子宫一氧化氮合酶(NOS)活力进行测定。结果 3个剂量组肝脏SOD活力、MDA含量比较,差异无统计学意义。高剂量组与低剂量组肾脏SOD活力、MDA含量比较,差异有统计学意义(P<0.05),随染毒NP浓度升高SOD活力降低,MDA含量升高。各剂量组子宫NOS活力比较差异有统计学意义(P<0.05),随染毒壬基酚浓度升高,NOS活力下降。结论 NP可导致雌性大鼠肾脏SOD活力降低、MDA含量升高,且导致子宫中NOS活力降低,说明壬基酚对肾脏及子宫造成了氧化损伤作用,对肝脏无氧化损伤作用。
Objective To observe the oxidative damage effect on the liver, kidney and uterus induced by nonylpheno! （NP） in female rats. Methods Twenty-four female SD rats were randomly divided into three groups, 8 in each, treated with NP at the doses of 0, 50 and 200 mg/kg respectively, through gavage. After 30 days of the treatment, the rats were sacrificed and the 10% homogenate of the liver, kidney and uterus were prepared for detection of SOD, MDA and NOS. Results There was no statistical difference in liver SOD activity and MDA content among the groups. In the kidney, the activity of SOD significantly declined （P〈0.01）, MDA content increased （P〈0.01）, and in the uterus, the activity of NOS significantly reduced （P〈0.01） as the exposure level of nonylphenol increased. Conclusion Exposure to nonylphenoi by oral route may cause oxidative damage in the kidneys and uterus in rats.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2010年第3期233-234,共2页
Journal of Environment and Health
关键词
壬基酚
氧化损伤
脂质过氧化
Nonylphenol
Oxidative damage
Lipid peroxidation
