摘要
本文对４种大肠杆菌菌株在不同生长时期的转化效率分别进行了测定．结果表明，在细菌的生长繁殖过程中，其转化效率是有很大变化的．对于不同的菌株，它们获得高转化率时的活菌数不同．同时，得到了这４种大肠杆菌菌株制备最佳感受态细胞的条件．
The efficiency of transformation of the competent cells, which were prepated from four strains of Escherichia coli , was determined respectively. The results showed that the efficiency of transformation could be altered in the process of growth and reproduction of bacteria. As to the higher efficiency of transformation, there were obvious differences in the virable bacterial density for different strains. And we set up the optimum condition for preparing the competent cells of the four strains.
出处
《生命科学研究》
CAS
CSCD
1998年第3期194-198,共5页
Life Science Research
基金
国家自然科学基金
关键词
大肠杆菌
感受态细胞
转化率
Escherichia coli , competent cell, efficiency of transformation
