摘要
提出了自动顶空-气相色谱-质谱联用检测血液中乙醇含量的方法。取一定量的血样或乙醇标准样品置于顶空瓶中（其中各加固定量的叔丁醇作为内标）,在60 ℃及中速振摇的条件下平衡20 min,通过进样环定量地将一定量样品引入于气相色谱-质谱联用仪器中进行测定。乙醇和叔丁醇的保留时间分别为1.47 min和1.56 min。方法的线性范围为0.01-10.00 g·L-1,检出限（3S/N）为0.6 mg·L-1,测定下限（10S/N）为1.0 mg·L-1。乙醇的回收率在92%-105%之间,测定值的相对标准偏差（n=6）小于7%。
A method for the determination of ethanol in blood by GC-MS with auto-headspace sample introduction was proposed. The blood sample or ethanol standard （together with a definite amount of tert. -butanol as internal standard） was placed in the head-space bottle and equilibrated at 60 ℃ for 20 rain with moderate-speed- shaking. Definite amount of sample was quantitatively introduced into the GC-MS instrument through the sample introduction loop. Retention time of ethanol and tert.-butanol was found at 1.47 rain and 1.56 min, respectively. Linear relationship between values of peak area and mass concentration of ethanol was obtained in the range of 0. 01--10. 00 g . L-1 with values of detection limit （3S/N） of 0. 6 mg . L-1 and lower limit of determination （10S/N） of 1.0 mg . L-1. The values of recovery of ethanol was found in the range from 92% to 105%, with RSD （n=6） less than 7%.
出处
《理化检验（化学分册）》
CAS
CSCD
北大核心
2010年第1期22-24,共3页
Physical Testing and Chemical Analysis(Part B:Chemical Analysis)
