摘要
从小麦叶片和穗部分离、筛选出对小麦赤霉病菌（Ｆｕｓａｒｉｕｍｇｒａｍｉｎｅａｒｕｍ）有很强拮抗作用的芽孢杆菌菌株Ｂ４、Ｂ６，经室内离体叶、穗防效测定和田间小区防效试验，二菌株对小麦赤霉病有较好防效，与清水对照相比，相对防效达６８４％、６８９％，接近多菌灵的防效（７０４％）。对Ｂ４、Ｂ６菌株进行形态和生理生化特征的分类鉴定，二菌株均为枯草芽孢菌（Ｂａｃｉｌｕｓｓｕｂｔｉｌｉｓ）
Biocontral agents of Bacillus spp. were isolated from the leaves and ears of wheat in different area. 300 isolates of Bacillus spp. were obtained and assayed for the antagonistic activity against Fusarium graminearium by agar-plate technique. Nine isolates presented the antagonistic activity (14 2～30 6mm inhibition zone). Two isolates B4, B6 with strong antagonistic activity were selected for further investigation in detail. The disease suppressing ability of B4, B6 strain was tested on detached-leaves and heads of wheat and in field plot exepriments by spraying suspenssion of B4, B6 before inoculation with conidia of F.graminearum. The results obtained from bioassay expriements show that the strain B4, B6 effectively controlled head blight caused by the pathogen. The effecacy of the two strains were 68 4% and 68 9% disease suppression sepratly, and very close to the control effecacy of Bavisitin fungicid (70 4% disease suppression). Strain B4, B6 appears to have excellent potential as biocontrol agents. Two strains were identified as Bacillus Sutilis species on the basis standard bacteria tests.
出处
《四川农业大学学报》
CSCD
1998年第3期314-318,共5页
Journal of Sichuan Agricultural University
