摘要
利用组织分离法,从自然发病的菊花病叶上分离了6株对菊花具有致病性的病原真菌。对获得的菌株进行了形态学鉴定,测定了不同菌株对菊花的致病力差异,并研究了一定浓度梯度范围内不同菌株分生孢子混合液和菌丝混合液人工接种的致病情况。结果表明,6个致病菌分别为链格孢属(Alternaria)的细极链格孢(A.te-nuissima)和链格孢(A.alternata)的不同菌株,各菌株对菊花叶片的致病力没有明显差异;菌株孢子混合液和菌丝混合液侵染保证100%发病的最低浓度分别为1 ml 1×106和1×107。A.tenuissima和A.alternata的不同菌株能够复合侵染菊花引发黑斑病。
Six pathogenetic fungi were isolated from infected leaves of chrysanthemum. Morphology of six isolates conidia was investigated for identification. Their potential pathogenicity causing black spot and inoculation on chrysanthemum using their condia mixture and mycelia mixture with different concentration were studied as well. The results showed that four of six isolates were A. tenuissima and the remaining two strains were A. alternata. There was no significant difference in their pathogenicity to chrysanthemum leaves. The minimum concentrations of condia and mycelia to cause disease rate 100% were 1 ml 1×10^6 and 1×10^7, respectively. Mixture of A. tenuissima and A. alternata could infect chrysanthemum causing leaf spot.
出处
《江苏农业学报》
CSCD
北大核心
2009年第4期752-757,共6页
Jiangsu Journal of Agricultural Sciences
基金
教育部新世纪优秀人才支持计划(NCET-06-0489)
国家科技支撑计划(2006BAD01A18)
江苏省高技术研究计划(BG2006315)
关键词
菊花
黑斑病
链格孢
人工接种
Dendranthema
grandiflorum
black spot
Alternaria
inoculation
